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| 食用百合卷丹离体培养再生体系的建立 | |
| 引用本文： | 张传海,葛自兵,白雪峰,孙婉.食用百合卷丹离体培养再生体系的建立[J].皖西学院学报,2012,28(2):1-3,6. |
| 作者姓名： | 张传海 葛自兵 白雪峰 孙婉 |
| 作者单位： | 1. 皖西学院生物与制药工程学院,安徽六安237012 安徽省植物生物技术实训中心,安徽六安237012 2. 安徽省舒丰现代农业科技开发有限公司,安徽舒城,231300 3. 安徽省植物生物技术实训中心,安徽六安,237012 |
| 基金项目： | 安徽省高校省级自然科学研究重点项目 |
| 摘 要： | 选取食用百合卷丹的鳞片作为外植体,以MS为基本培养基,添加6-BA和NAA的不同植物激素组合,分组进行不定芽诱导、继代增殖和诱导生根培养。结果表明,鳞茎的中层鳞片具有较强的不定芽分化能力,6-BA 0.5mg/L＋NAA 0.1mg/L的激素组合适宜于鳞片的不定芽分化和继代增殖,而最利于诱导生根的激素组合为NAA 1.0mg/L＋6-BA 0.1mg/L。组培苗经过60天左右培养可在培养基中直接产生小鳞茎。 |
| 关 键 词： | 百合卷丹 鳞片 不定芽 快速繁殖 |
Establishment of Regeneration Systems by Squama Culture of Lilium Lancifolium Thunb |
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| ZHANG Chuan-hai,GE Zi-bing,BAI Xue-feng,SUN Wan.Establishment of Regeneration Systems by Squama Culture of Lilium Lancifolium Thunb[J].Journal of Wanxi University,2012,28(2):1-3,6. | |
| Authors: | ZHANG Chuan-hai GE Zi-bing BAI Xue-feng SUN Wan |
| Institution: | 1.College of Biological and Pharmaceutical Engineering,West Anhui University,Lu＇an 237012,China; 2.Anhui Shufeng Modern Agriculture Science and Technology Development Limited Company, Shucheng 231300,China;3.Plant Biotechnology Training Center of Anhui Province,Lu＇an 237012,China) |
| Abstract: | Squamas of edible lily bulbs of Lilium lancifolium Thunb were used as explants,and MS media added different combination of 6-BA and NAA were employed for adventitious bud induction,bud multiplication subculture and root induction.The results showed that bud differentiation ability of outward squamas was better than that of inward ones,the medium MS ＋ 6-BA 0.5 mg/L ＋ NAA 0.1 mg/L was optimal both to bud induction and to bud multiplication,and the optimal medium for rooting was MS ＋ NAA 1.0 mg/L ＋ 6-BA 0.1 mg/L.Small bulbs directly generated from seedlings after an about 60 days＇ continuous culture in the medium. |
| Keywords: | Lilium lancifolium Thunb bulb squamas adventitious buds rapid propagation |
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