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A群链球菌制剂的制备工艺
引用本文:郎维,张峰,白春喜,邱芳萍.A群链球菌制剂的制备工艺[J].吉林工学院学报,2008,29(6):647-652.
作者姓名:郎维  张峰  白春喜  邱芳萍
作者单位:长春工业大学化学与生命科学学院,吉林长春130012
基金项目:吉林省科技计划发展基金资助项目  
摘    要:采用改良后的无血培养基,将筛选选育后得到的A群链球菌进行制剂的制备研究,以发酵后菌悬液的OD值为指标,通过正交试验分析对其进行条件优化。得到的制备工艺为:在37℃、摇床转速140r/min下,装瓶量150mL、pH值7.5、酵母粉用量0.5%、胰蛋白胨用量1.5%、牛肉膏用量0.4%、葡萄糖用量0.5%。实验表明,菌体在改良后的培养基上生长良好,形态特征符合A群链球菌的形态特征。

关 键 词:A群链球菌  无血培养基  制备工艺

The preparation techniques of Group A streptococcus
LANG Wei,ZHANG Feng,BAI Chun-xi,QIU Fang-ping.The preparation techniques of Group A streptococcus[J].Journal of Jilin Institute of Technology,2008,29(6):647-652.
Authors:LANG Wei  ZHANG Feng  BAI Chun-xi  QIU Fang-ping
Institution:School of Chemical & Life Science;Changchun University of Technology;Changchun 130012;China
Abstract:With the improved bloodless culture medium, the cultivated Group A streptococcus is prepared. The fermentation conditions are to be optimized through the orthogonal experiment by using OD660 value of fermented bouillon as the index. The optimized conditions are: temperature 37 ℃, the rotate speed of the vibrator 150r/min, the solution volume 150ml, pH value 7. 5, the dosage of yeast 0.5%, the dosage of tryptone 1.5%, the dosage of beef extract 0.4% , the dosage of glucose 0. 5%. The results show that the bacterial grow well in the improved medium and the morphological characteristics are consisted with that of Group A streptococcus.
Keywords:Group A streptococcus  bloodless culture medium  preparation technique    
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