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| Performance of a Pectinase from Bacillus Subtilis WSHB04 - 02 Used in Bioscouring of Cotton Fabrics | |
| 作者姓名： | 王强 范雪荣 陈坚 |
| 作者单位： | [1]Key Laboratory of Eca-Textiles, Ministry of Education, Southern Yangtze University, Wuxi, Jiangsu 214122, China [2]Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University ,Wuxi, Jiangsu 214036 ,China [3]School of Biotechnology, Southern Yangtze University, Wuxi, J iangsu 214036, China |
| 基金项目： | 国家高技术研究发展计划(863计划) , 江苏省自然科学基金 , Open Project Program of Key Laboratory of Eco-Textiles(Southern Yangtze University), Ministry of Education, China |
| 摘 要： | A pectinase produced by Bacillus subtilis WSHB04-02 isolated from soil with lyase activity operating at alkaline pH was studied. The MichaelisMenten kinetic parameters of this newly isolated pectinase on different substrates, such as citrus pectin and polygalacturonic acid （PGA）, were determined, and pectin proved to be the most suitable substrate. The effects of temperature and pH on pectinase activity and stability were also investigated. The optimal temperature for pectinase was 55℃ with a stable range of 45℃- 55℃. In general, pectinase was pH insensitive and the stable pH ranged from 8.6 to 10.0. Ultimately the bioscouring effects of cotton fabrics using this pectinase were evaluated and some promising results were obtained. |
| 关 键 词： | 果胶酶 活性 稳定性 棉花 |
| 文章编号： | 1672-5220（2007）02-0178-05 |
| 修稿时间： | 2006-08-20 |
Performance of a Pectinase from Bacillus Subtilis WSHB04-02 Used in Bioscouring of Cotton Fabrics |
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| WANG Qiang,FAN Xue-rong,CHEN Jian.Performance of a Pectinase from Bacillus Subtilis WSHB04 - 02 Used in Bioscouring of Cotton Fabrics[J].Journal of Donghua University,2007,24(2):178-182. | |
| Authors: | WANG Qiang FAN Xue-rong CHEN Jian |
| Institution: | 1. Key Laboratory of Eco-Textiles, Ministry of Education, Southern Yangtze University,Wuxi,Jiangsu 214122,China;Key Laboratory of Industrial Biotechnology,Ministry of Education,Southern Yangtze University,Wuxi, Jiangsu 214036,China 2. Key Laboratory of Eco-Textiles, Ministry of Education, Southern Yangtze University, Wuxi, Jiangsu 214122,China 3. Key Laboratory of Industrial Biotechnology,Ministry of Education,Southern Yangtze University,Wuxi, Jiangsu 214036,China;School of Biotechnology,Southern Yangtze University,Wuxi, Jiangsu 214036,China |
| Abstract: | A pectinase produced by Bacillus subtilis WSHB04-02 isolated from soil with lyase activity operating at alkaline pH was studied. The MichaelisMenten kinetic parameters of this newly isolated pectinase on different substrates, such as citrus pectin and polygalacturonic acid (PGA), were determined, and pectin proved to be the most suitable substrate. The effects of temperature and pH on pectinase activity and stability were also investigated. The optimal temperature for pectinase was 55℃ with a stable range of 45℃- 55℃. In general,pectinase was pH insensitive and the stable pH ranged from 8.6 to 10.0. Ultimately the bioscouring effects of cotton fabrics using this pectinase were evaluated and some promising results were obtained. |
| Keywords: | pectinase activity stability bioscouring cotton |
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