鬼箭锦鸡儿细胞毒活性成分研究

 运用中压柱色谱和高效制备液相色谱方法对藏药鬼箭锦鸡儿的化学成分进行分离纯化,利用现代波谱技术鉴定所分离化合物的结构,并对所得到的化合物进行体外抗肝癌活性测试.从鬼箭锦鸡儿乙醇提取物中分离得到8个单体化合物,分别鉴定为6,7,3′-三羟基-4′-甲氧基异黄酮（1）、3,7-二羟基-4′-甲氧基黄酮（2）、3-甲氧基-4,9-二羟基紫檀素（3）、7,3′-二羟基-5′-甲氧基异黄酮（4）、6,7-二羟基-4′-甲氧基异黄酮（5）、3,9-二甲氧基-8-羟基紫檀素（6）、3,9-二甲氧基-4-羟基紫檀素（7）和3-甲氧基-4-羟基高丽槐素（8）,所有的化合物为首次从该植物中分离得到.采用MTT法对化合物1～8进行体外抗肝癌活性测试,结果显示所有的化合物对2个肝癌细胞株HepG2和Hep3B均有抑制作用,它们的IC50值范围为22.5～104.7μg/mL.

Cytotoxic chemical constituentsfrom Caragana jubata(pall)Poir.

To discover new cytotoxic constituents from the traditional tibetan medicine-“Zuomaoxing”(roots of Caragana jubata (pall) Poir.),the isolation and purification of a methanolic extract was performed with medium-pressure column chromatography and preparative HPLC. Eight pure compounds were obtained and their structures were elucidated as 6,7,3′-trihydroxy-4′-methoxyisoflavone (1), 3,7-dihydroxy-4′-methoxyflavonone (2),3-methoxy-4,9-dihydroxy pterocarpan (3),7,3′-dihydroxy-5′-methoxyisoflavone (4),6,7-dihydroxy-4′-methoxyisoflavone (5),3,9-dimethoxy-8-hydroxy pterocarpan (6),3,9-dimethoxy-4-hydroxy pterocarpan (7) and 3-methoxy-4-hydroxy maackiain (8) based on detailed spectral analysis.All compounds were isolated from the title plant for the first time.In vitro cytotoxic activity of compounds 1—8 were evaluated using MTT method,and the results showed that all compounds exhibited cytotoxic activity against HepG2 and Hep3B cell lines with IC50 values ranging from 22.5 to 104.7μg/mL. 