木霉No.183菌株木聚糖酶的研究

 筛选到一株木聚糖酶高产木霉菌株(No.183),研究了该菌株产木聚糖酶的液态发酵和粗酶液的酶学性质.结果表明,以麸皮和木聚糖为主要碳源,28℃,190r/min摇瓶培养时,木霉No.183菌株在接种后84h酶活最高,达到298.47U/mL.该木聚糖酶的最适反应温度为50℃,最适pH为该木聚糖酶在pH5～7和40℃以下时相对稳定.Ca²⁺,Zn²⁺和Cu²⁺对该木聚糖酶有较强的促进作用,Fe³⁺和Hg²⁺对该酶有较强的抑制作用.

Studies on the crude xylanase produced by Trichoderma sp. No.183

The enzymological characteristics of crude xylanase produced by Trichoderma strain 183 with high xylanase productivity were studied with the following results:cultured on the medium with wheat bran and xylan as the main carbon resource,under 28℃ and 190r/min shaking,the pH and enzyme activity of the broth kept increasing with the growth of strain 183,the enzyme activity reached its peak at hour 84 after inoculation,up to 298.47U/mL.The enzyme has optinal reactive conditions of 50℃ and pH 6,though the enzyme is relatively stable under 40℃ and pH 5～7.Fe²⁺ and Hg²⁺ strongly inhibited the activity of this enzyme,while Zn²⁺,Ca²⁺ and Cu²⁺ evidently promoted the activity.