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| pH色谱法测定灯盏花乙素离解常数 | |
| 引用本文： | 张伟,闫娟娟,杨兆祥.pH色谱法测定灯盏花乙素离解常数[J].云南大学学报(自然科学版),2010,32(2):196-200. |
| 作者姓名： | 张伟 闫娟娟 杨兆祥 |
| 作者单位： | 1. 昆明制药集团股份有限公司药物研究院, 云南昆明 650100; 2. 大理学院, 云南大理 671003 |
| 摘 要： | 通过高效液相色谱(HPLC)和薄层色谱法(TLC)对灯盏花乙素离解常数进行测定方法研究.在薄层色谱方法中,利用待测组分比移值(Rf值)与固定相pH值的Rf-pH曲线方程;在高效液相色谱方法中,利用待测组分保留因子(κ值)与流动相pH值的κ-pH曲线方程,测定了灯盏花乙素的离解常数,并比较了HPLC和TLC法对灯盏花乙素离解常数的测定.HPLC与TLC法对灯盏花乙素离解常数的测定中κ-pH曲线和Rf-pH曲线均符合S型数理模型,该文首次报道了灯盏花乙素离解常数及测定方法.用所建立的方法分别测定了灯盏花乙素的离解常数并进行了比较,HPLC法准确度高,重现性好,方便简单,分析快速、准确. |
| 关 键 词： | 灯盏花乙素 高效液相色谱法 薄层色谱法 离解常数 测定 |
| 收稿时间： | 2009-9-3 |
Determination of dissociation constant of scutellarin by thin-layer pH-chromatography and HPLC pH-chromatography |
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| ZHANG Wei,YAN Juan-juan,YANG Zhao-xiang.Determination of dissociation constant of scutellarin by thin-layer pH-chromatography and HPLC pH-chromatography[J].Journal of Yunnan University(Natural Sciences),2010,32(2):196-200. | |
| Authors: | ZHANG Wei YAN Juan-juan YANG Zhao-xiang |
| Institution: | 1. Institute for Drug Research and Development of Kunming Pharmaceutical Corporation, Kunming 650100, China; 2. Dali University, Dali 671003, China |
| Abstract: | Objective:To determine the dissociation constant of scutellarin by thin-layer pH-chromatography and HPLCpH-chromatography.Method:The dissociation constant of scutellarin is calculated respectively from the data of Rf-pHcurves in the thin-layer pH-chromatography,and the retention factors-pH of mobile phase in the HPLCpH-chromatography.Results:The curves of the two methods showed to be suitable for sigmoidal fit.The dissociation constant of scutellarin and its determination were studied for the first time.Conclusion:Compared the thin-layer pH-chromatography with the HPLCpH-chromatography,the method of HPLCph-chromatography is more repetitive,rapid and accurate. |
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