| 昆明地区1株HAM野毒株的基因分型 |
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| 引用本文： | 井申荣,姜述德,余芬.昆明地区1株HAM野毒株的基因分型[J].云南大学学报(自然科学版),2001,23(1):77-80. |
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| 作者姓名： | 井申荣 姜述德 余芬 |
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| 作者单位： | 中国医学科学院 中国协和医科大学 医学生物学研究所, |
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| 基金项目： | 云南省自然科学基金资助项目（1999C0023Q）. |
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| 摘 要： | 免疫捕获粪便悬液中的HAV，RT－PCR扩增目的的片段，将其连接到载体pUC18中，测序并对序列进行分析和比较，HAV野毒株W在核苷酸3024－3191与HM175/wt序列相同，表明W株为ⅠB亚型；该株5'NTR的核苷酸29－258与其它国际毒株有很大的不同，有碱基的、缺失和插入，其中插入的15个碱基是其它毒株所没有的。
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| 关 键 词： | 甲肝病毒 野毒株 基因分型 昆明地区 HAV野毒株 RT－PCR扩增 序列分析 |
| 文章编号： | 0258-7971(2001)01-0077-04 |
| 修稿时间： | 2000年11月28 |
Genetype of A Wild-type
HAV Strain in Kunming |
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| JING Shen-rong,JIANG Shu-De,Yu Fen.Genetype of A Wild-type
HAV Strain in Kunming[J].Journal of Yunnan University(Natural Sciences),2001,23(1):77-80. |
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| Authors: | JING Shen-rong JIANG Shu-De Yu Fen |
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| Abstract: | HAV particles were captured from clinical fecal suspension by immunoaffinity.The viral fragment synthesized by RT-PCR was recombined into vector pUC18,and analyzed after sequencing.The sequence of HAV wild-type W is the same as HM175/wt at nt3024—3191,it showed that strain W is sub-type ⅠB.However,substitutions,deletion and insertions in 5'NTR at nt58—294 of HAV strain W were detected as compared to other strains isolated from different countries.The inserted 15 bases were not found in any other HAV strains. |
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| Keywords: | HAV wild type strain genetypeP |
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