不同产地不同部位云黄连生物碱成分累积研究

 研究不同产地云黄连不同部位中盐酸小檗碱、药根碱和巴马汀3种生物碱积累量差异,旨在寻找云黄连优质产区及为非药用部位综合利用提供理论依据.在云黄连采收期,分别采集泸水、贡山、福贡、腾冲4县共10个地区的云黄连居群,测定云黄连根茎、须根和叶片3个部位的生物量,采用HPLC法测定样品不同部位中盐酸小檗碱、药根碱和巴马汀的含量.结果显示,云黄连不同部位3种生物碱含量大小依次为根茎>叶>须根.云黄连根茎中3种生物碱在不同产地间差异显著,其中贡山县后山、贡山县丙中洛神山、贡山县普拉底乡3个地区所产云黄连生物碱含量、生物量、生物积累量较高.其中贡山县普拉底乡积累量最高,其盐酸小檗碱、药根碱和巴马汀单株平均积累量分别为46.899,2.76,1.708 mg.结论:在云黄连种植生产过程中,在考察其有效成分含量的同时,还应考虑其生物量.综合比较不同产地不同部位生物量、3种生物碱含量及其积累量等因素,确定贡山县后山、贡山县丙中洛神山、贡山县普拉底乡为云黄连优质产区.非药用部位中3种生物碱的积累量较大,应收集后进行有效成分的提取,达到云黄连资源综合利用的目的.

Investigation of alkaloids contents in various organs of Coptis teeta Wall grown in different areas

The contents of three kind of alkaloids(berberine,jateorhizine,palmatine) in various organs of Coptis teeta Wall grown in different areas were investigated to screen the best production areas and to make use of three kind of alkaloids containing in non-officinal organ of Coptis teeta Wall.Method:Comparison and analysis of the biomass(rhizome,fibrous root,leaf) and the three kind of alkaloids contents from 10 different production areas were performed.Result:The differences of three kind of alkaloids among different organs and different production areas were significant,the order of the contents of the three kind of alkaloids was:rhizome > leaf>fibrous root.The quality of Coptis teeta Wall grown in Houshan of Gongshan County,Bingzhongluo village of Gongshan County and Puladi Village of Gongshan County was the best.Conclusion:The three production areas mentioned above were the best areas for growing Coptis teeta Wall.The 3 alkaloids in non-officinal organ of Coptis teeta Wall should be used.