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| 壳聚糖荧光标记反应研究 | |
| 引用本文： | 范妮,薛伟明.壳聚糖荧光标记反应研究[J].云南大学学报(自然科学版),2010,32(1):77-81. |
| 作者姓名： | 范妮 薛伟明 |
| 作者单位： | 1. 陕西教育学院化学系, 陕西西安 710061; 2. 西北大学化工学院, 陕西西安 710069 |
| 摘 要： | 以壳聚糖-海藻酸钙微球在体检测为研究背景,建立壳聚糖的荧光标记方法.以荧光素异硫氰酸酯(FITC)作为标记化合物,采用紫外-分光光度计在447nm下检测标记在壳聚糖上的FITC,通过m(FITC,μg)/m(壳聚糖,g)表征壳聚糖的荧光标记效率.考察壳聚糖溶液pH值、浓度、反应时间以及壳聚糖分子量、脱乙酰度对壳聚糖荧光标记反应的影响.结果表明:选取相对分子质量20万、脱乙酰度95%的壳聚糖配制成0.01g/mL的溶液,在pH10的条件下与荧光素反应5h将制得标记效率为117.4μg/g的荧光壳聚糖.建立了一种稳定有效的壳聚糖荧光标记方法,为荧光标记壳聚糖-海藻酸钙微球提供了技术支持. |
| 关 键 词： | 荧光素 标记 壳聚糖 |
| 收稿时间： | 2009-4-20 |
Study on label reaction of chitosan with fluorescein |
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| FAN Ni,XUE Mei-ming.Study on label reaction of chitosan with fluorescein[J].Journal of Yunnan University(Natural Sciences),2010,32(1):77-81. | |
| Authors: | FAN Ni XUE Mei-ming |
| Institution: | 1. Department of Chemistry, Shaanxi Institute of Education, Xi'an 710061, China; 2. College of Chemical Engineering, Northwest University, Xi'an 710069, China |
| Abstract: | A effective and stable method to label chitosan with fluorescein isothiocyanate (FITC) was established in order to detect chitosan-calcium alginate microspheres in vivo.The fluorescence intensity of FITClabeled chitosan was detected at 447 nm by UV-spectrophotometer and the efficiency of FITClabel chitosan was characterized bymFITC(μg) /mchitosan(g).It was studied that the effect of pH,concentration,reaction time,the molecular weight and deacetylation degree of chitosan on label reaction.The content of FITCon chitosan was determined to be 117.4 μg/g after reaction for 5hrs with 0.01 g/mLchitosan (200000 dalton,degree of deacytylation 95% ) at pH10. |
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