HBs和HCVc基因双顺反子质粒在BALB/c小鼠体内的分布

 采用注射含双顺反子质粒后,PCR法扩增不同组织中HBsAg和HCVc基因,同时检测HBV和HCV抗体应答水平.研究注射基因免疫用双顺反子质粒在小鼠组织中的分布.pcDNA3.0BApc154S₂S 1次注射BALB/c小鼠后,24 h内主要分布于血液、肝、脾、骨髓、淋巴结、注射部位肌肉、肺和肾等含血液丰富的组织中.注射后2 d主要在骨髓、血液和注射部位肌肉中存在.7 d后仅在注射部位肌肉中能检测出来,并可持续到第9周.组织切片镜检质粒注射初期肌肉细胞轻度浊肿,随后肌膜细胞轻度增生,未见其它明显的组织病理学变化.质粒多次免疫BALB/c小鼠未见明显的临床症状和病理组织学变化.质粒在小鼠体内不同组织存在时间不一致.

Tissure distribution in BALB/c of bicistronic plasmids HCV with core and HBV preS2S

DNA immunization can induce humoral and cellular immune responses to against a range of viral pathogens.The results show HCV core and HBV preS_2S can induce strong immune responses in DNA immunization.After muscular adminstration was performed,pc154S_2S plasmid DNA was detected initially in all highly vascularized tissues.Two days latter,they mainly stayed at span,blood and injceted muscles;Seven days latter,the pc154S_2S only can be found in the injected muscles,it can be kept for nine weeks.Intramuscular injections of plasmid has no clinical symptom and histopathogical damages in the injection sites.