首页 | 本学科首页   官方微博 | 高级检索  
     检索      

固相萃取和高效液相色谱法测定鱼腥草中的黄酮
引用本文:杨亚玲,周强,刘东辉,赵榆林.固相萃取和高效液相色谱法测定鱼腥草中的黄酮[J].云南大学学报(自然科学版),2006,28(2):157-160.
作者姓名:杨亚玲  周强  刘东辉  赵榆林
作者单位:昆明理工大学,生命与化学工程学院,云南,昆明,650224
基金项目:教育部重点项目资助(地方02164)
摘    要: 研究了用固相萃取预分离,高效液相色谱法测定鱼腥草根和鱼腥草叶中黄酮类物质的方法.鱼腥草根和鱼腥草叶中的黄酮用80%乙醇溶液加热回流提取,提取液用Waters Sep-Pak-C18固相萃取小柱预分离脱脂,以Waters Nova-Pak-C18(Φ3.9 mm×150 mm,5μm)色谱柱为固定相,以0.05 mol/L磷酸二氢钾缓冲溶液和甲醇的体积比为40:60的混合液为流动相,在该色谱条件下,鱼腥草中主要的黄酮成分均达到基线分离;用紫外二极管矩阵检测器在360 nm波长处检测,并作了色谱峰纯度分辨.方法标准回收率为98%~101%,相对标准偏差为0.85%~2.2%.

关 键 词:高效液相色谱  固相萃取  鱼腥草  黄酮
文章编号:0258-7971(2006)02-0157-04
修稿时间:2005年6月30日

Study on determination of flavonoid in houttuynia cordata thunb by solid phase extraction and RP-HPLC
YANG Ya-ling,ZHOU Qiang,LIU Dong-hui,ZHAO Yu-lin.Study on determination of flavonoid in houttuynia cordata thunb by solid phase extraction and RP-HPLC[J].Journal of Yunnan University(Natural Sciences),2006,28(2):157-160.
Authors:YANG Ya-ling  ZHOU Qiang  LIU Dong-hui  ZHAO Yu-lin
Institution:College of Biologic and Chemical Engineering, Kunming University of Science and Technology, Kunming 650224, China
Abstract:A high performance liquid chromatography(HPLC) method for the determination of flavonoid in myrica rubra leaf and bark was studied.The flavonoid was extracted from the sample with 80% of ethanol by heating circumfluence.Then the extract was degreased by solid phase extraction with Sep-Pak-C18 cartridge.The flavonoid was separated on a Nova-Pak-C18 chromatogram column(3.9×150mm,5μm),with methanol and 0.05mol/L potassium dihydrogen phosphate buffer solution as mobile phase at 60:40(V/V),and monitored with the photodiode array detector at 360nm.The recoveries of the flavonoid are 98%-101%,and relative standard deviations are 0.85%-2.2%.
Keywords:RP-HPLC  solid phase extraction  houttuynia cordata thunb  flavonoid
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《云南大学学报(自然科学版)》浏览原始摘要信息
点击此处可从《云南大学学报(自然科学版)》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号