乔木茵芋中黄酮和生物碱类化合物及其镇痛活性研究

 对乔木茵芋进行化学成分研究.采用硅胶柱色谱、葡聚糖凝胶LH-20柱色谱、RP-Csub>18柱色谱等方法分离化学成分,¹H NMR、¹C NMR等方法进行结构鉴定,从中分离鉴定了5个黄酮类化合物:槲皮素(1),槲皮苷(2),山奈酚-3,7-葡萄糖苷(3),枸橘苷(4),橙皮苷(5);1个生物碱类化合物:1-甲基-2-苯基-4-喹啉酮(6).所有化合物均为首次从该种植物中分离得到.并采用小鼠醋酸扭体法和热板法检测乔木茵芋总提取物及乔木茵芋总生物碱提取物的镇痛活性.结果显示乔木茵芋总提物和乔木茵芋总生物碱提取物均可明显抑制醋酸所致小鼠扭体反应,此外乔木茵芋总生物碱提取物还可明显提高小鼠对热刺激的痛阈值,具有较好的镇痛活性.

Studies on flavonoid and alkaloid from Skimmia arborescens Anders. ex Gamble and its pain releasing effect

To study the chemical constituents of Skimmia arborescens.The chemical constituents were isolated by silica gel column chromatography,Pharmadex LH-20,RP-Csub>18,and the ¹H NMR,¹C NMR spectroscopic analysis were employed for the structural elucidation.Six compounds were isolated from Skimmia arborescens.Their structures were elucidated as quercetin(1),quercetin 3-O-α-L-Rha(2),kaempferol-3,7-o-β-D-glucosides(3),poncirin(4),hesperidin(5),1-methyl-2(Z)-7-phenyl]-4(1H)-quinolone(6),The 6 compounds were isolated from the plant for the first time.Using acetic acid writhing and hot plate test in mice,the pain releasing effects of the total extract and the total alkaloids from the plant were evaluated.The results indicated that both the total extract and total alkaloids of Skimmia arborescens could markedly decrease mice writhing caused by acetic acid.Furthermore,the total alkaloid of Skimmia arborescens could extend evidently pain threshold in mice.