滇藏地区8种鹅膏菌的ITS序列分析

 对滇藏地区8种鹅膏菌的核糖体DNA(rDNA)内转录间隔区(ITS)进行PCR扩增和测序,用ClustalX(version 1.81)软件对所测的ITS序列进行比对分析.结果表明,除5.8S rDNA(其长度为160 bp)比较保守外,8个不同种的ITS序列在长度和碱基位点上都存在较大差异,其中ITS1区间的片段长度为206～297 bp,ITS2区间的片段长度为191～238 bp.这说明鹅膏属的种间具有较高的遗传多样性.利用MEGA(version 3.1)软件进行聚类,8个鹅膏菌在D=0.14处分成3组,与传统分类有出入.这为鹅膏菌的进一步分类及研究提供了分子依据.

ITS sequence analysis of 8 Amanita species from Tibetan and Yunnan

The internal transcribed spacer(ITS) regions of ribosomal DNA of 8 Amanita species from Tibetan and Yunnan in China were sequenced by PCR amplication,and their sequences were analyzed by the software of Clustal X(version 1.81).The results showed that,the ITS sequences of 8 species had significant differences in both length and bases,besides the fragment of 5.8S rDNA,whose length is 160bp,being relatively conservative;the length of these fragments of ITS1,ITS2,were 206—297bp,191—238bp,respectively.These implied that the genetic diversity among the Amanita species was relatively high.The 8 Amanita species was dividied into 3 groups at D=0.14 according to the phylogenitic analysis by MEGA and it was dissimilar to traditional taxonomy.The results above provided a molecular reference for further taxonomy and study of Amanita in the future.