| 应用重组Etp28抗原免疫预防球虫病的研究 |
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| 引用本文： | 吴秋豫,杨林,左仰贤.应用重组Etp28抗原免疫预防球虫病的研究[J].云南大学学报(自然科学版),2000,22(2):150-153. |
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| 作者姓名： | 吴秋豫 杨林 左仰贤 |
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| 作者单位： | 1. 中山大学,生命科学学院,广东,广州,510275
2. 云南大学,生物系,云南,昆明,650091 |
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| 基金项目： | 国家自然科学基金!( 3 980 0 10 8)，云南省应用基础研究基金资助项目!( 94C0 5M ) |
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| 摘 要： | 将构建好的可表达ＧＳＴ融合蛋白的重组病毒ＡｃＭＮＰＶ－ＯＣＣ－Ｅｔｐ２８感染Ｓｆ９细胞，７２ｈ后取感染了病毒的细胞裂解物上清液进行ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｍ－ｂｌｏｔ分析，结果显示５３ｋＤａ的融合蛋白在昆虫Ｓｆ９细胞中得到了高效表达，表达水平为占细胞总蛋白的２１．３％；将ＧＳＴ－６ｘＨｉｓ－Ｅｔｐ２８注射维菌鸡进行免疫保护试验，结果表明免疫组比对照组在平均增重和盲肠粘膜层卵囊计数方面有一定程度
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| 关 键 词： | Etp28重组抗原 球虫病 免疫保护 家禽 疫苗 家畜 |
| 修稿时间： | 1999-11-23 |
Study on the Coccidiosis Prevention with Recombinant Etp28 Antigen |
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| WU Qiu-yu,YANG Lin,ZUO Yang-xian.Study on the Coccidiosis Prevention with Recombinant Etp28 Antigen[J].Journal of Yunnan University(Natural Sciences),2000,22(2):150-153. |
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| Authors: | WU Qiu-yu YANG Lin ZUO Yang-xian |
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| Abstract: | The recombinant viruses,AcMNPV OCC - GST 6xHis Etp28,obtained by co transfection,were used to transfect the Sf9 cells,and the supernatants of 72?h pi cell lysate was examined by SDS PAGE and Western blot.Results showed GST 6xHis Etp28 fusion protein of 53?kDa was expressed.Densitometric scanning of SDS PAGE gel revealed the expressed fusion protein accounted for 21.3% of the total cell proteins.GST 6xHis Etp28 was injected into chicken muscle to detect the immunoprotective effect of recombinant antigen.The results showed that immunized group had some improvement on wight increment and oocyst quantity of chicken cecal musosa. |
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| Keywords: | recombinant antigen of Etp28 baculovirus expression system coccidiosis immune protection |
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