高效液相色谱法测定果酒中的糖、甘油和乙醇

 研究了高效液相色谱法测定果酒中的糖、甘油和乙醇的方法.果酒样品用Sep-Pak-C18固相微萃取小柱预分离,以WatersSugar-PakⅠ钙型阳离子交换柱为固定相,0.05g/LEDTA钙钠水溶液为流动相,示差折光仪为检测器,一次进样测定果酒样品中的蔗糖、葡萄糖、果糖、甘油和乙醇.最低检测限在微克级,相对标准偏差在0.92%～1.52%之间,标准回收率在97%～105%之间.方法用于果酒样品的测定,结果满意.

Determination of sugar, glycerol and ethanol in ratafee with high performance liquid chromatography

A high performance liquid chromatography(HPLC) method for the determination of sugar,glycerol and ethanol in ratafee was studied.Ratafee was preseparated by solid phase extraction with Sep Pak C 18 column.Sugars,glycerol and ethanol were separated on a Waters Sugar Pak Ⅰ(6.5mm×300mm) cations ion exchange column,with a refractive index detector system and 0.05g/L EDTA calcium disodium salt solution as mobile phase.Sucrose,glucose,fructose,glycerol and ethanol could be simultaneously determined in a single injection.The limits of detection were:sucrose 1.5μg/mL,glucose 2.0μg/mL,fructose 2.0μg/mL,glycerol 2.5μg/mL and ethanol 2.5μg/mL.The mean recoveries of sugar,glycerol and ethanol were between 97% and 105% and the relative standard deviations were between 0.92%and 1.52%.This method can be applied to the determination of sugars,glycerol and ethanol in ratafee with satisfactory result.