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木霉菌对烟草黑胫病菌的拮抗机制及其生物防治研究
引用本文:王革,李梅云,段玉琪,李松,马永凯,王颖琦,朱维明,李振国,李天飞.木霉菌对烟草黑胫病菌的拮抗机制及其生物防治研究[J].云南大学学报(自然科学版),2001,23(3):222-226.
作者姓名:王革  李梅云  段玉琪  李松  马永凯  王颖琦  朱维明  李振国  李天飞
作者单位:1. 云南玉溪红塔集团技术中心,
2. 云南烟草科学研究院农业所,
摘    要:利用诱捕法从土壤中分离得到1株木霉菌株长枝木霉(Trichoderma longibrachiatum),采用对峙法测定其对云南省烟草黑胫病菌(Phytophthora nicotiana)的拮抗作用,结果表明:该菌对黑胫病菌具较强拮抗作用,拮抗机制主要为竞争作用,重寄生作用,抗生作用,采用温室盆栽,田间试验,分别测定该 对黑胫病的防效,室内防效为97.6%(10^6个/mL浓度级),田间移栽时,每株施用4克效果较好,田间防效可达67%以上。

关 键 词:长枝木霉  烟草黑胫病  拮抗机制  生物防治  防效  真菌病害  木霉菌
文章编号:0258-7971(2001)03-0222-05
修稿时间:2001年2月8日

Study on the Antaganistic Mechanism of Trichoderma sp. for Phytophthora Nicotianae and it's Biocontrol Effection
WANG Ge ,LI Mei yun ,DUAN Yu qi ,LI Song ,MA Yong kai ,WANG Ying qi ,ZHU Wei ming ,LI Zheng guo ,LI Tian fei.Study on the Antaganistic Mechanism of Trichoderma sp. for Phytophthora Nicotianae and it's Biocontrol Effection[J].Journal of Yunnan University(Natural Sciences),2001,23(3):222-226.
Authors:WANG Ge  LI Mei yun  DUAN Yu qi  LI Song  MA Yong kai  WANG Ying qi  ZHU Wei ming  LI Zheng guo  LI Tian fei
Institution:WANG Ge 1,LI Mei yun 2,DUAN Yu qi 2,LI Song 1,MA Yong kai 1,WANG Ying qi 1,ZHU Wei ming 1,LI Zheng guo 1,LI Tian fei 2
Abstract:One isolate of Trichoderma sp.was obtained by the method of baiting.The antagonistic test of the isolate to strains of Phtophthora nicotiana (pathogen of tobacco black shank) from diseased tobacco stems from different area of Yunnan were carried out by pairing cuture on PDA.The results showed that this isolate was strong antagonistic to black shank fungus.The antagonistic mechanism appeared to be competitive growth and antibiosis.The control effect of this isolate applied for tobacco black shank in greenhouse was as high as 97.6%(10 6?cfu/mL).When applied in field soil around the tobacco root at 4?g every root,the control effect could arrive at 67%.
Keywords:Trichoderma longibrachiatum  tobacco black shank  antagonistic mechanism  biocontrol  control effect
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