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| 苯酚降解菌的富集、分离与鉴定 | |
| 引用本文： | 刘旭光,邵世光.苯酚降解菌的富集、分离与鉴定[J].云南大学学报(自然科学版),2009,31(2):195-199. |
| 作者姓名： | 刘旭光 邵世光 |
| 作者单位： | 1. 连云港师范高等专科学校连云港市应用生物技术重点实验室, 江苏 连云港, 222006; 2. 南京大学, 生命科学学院, 江苏 南京, 210093; 3. 南京师范大学, 生命科学学院, 江苏 南京, 210046 |
| 摘 要： | 用苯酚作为唯一碳源富集培养化工厂、废水处理厂、造纸废水样品,从中分离得到4株具有苯酚降解能力的菌株.用编码苯酚羟化酶大亚基基因的保守序列设计特异引物进行检测,共有3株菌扩增出相应的产物.对其中降解能力最强的1株菌的16SrDNA序列进行系统发育分析,确定其为假单胞菌.与以前方法相比,本方法可检测到目前几乎所有的苯酚羟化酶基因,从而实现快速、准确、方便地从苯酚污染的环境中检测苯酚降解菌. |
| 关 键 词： | 苯酚降解菌 苯酚羟化酶基因 假单胞菌 PCR检测 |
| 收稿时间： | 2008-11-11 |
Enrichment,isolation and identification of phenol degrading bacteria |
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| LIU Xu-guang,SHAO Shi-guang.Enrichment,isolation and identification of phenol degrading bacteria[J].Journal of Yunnan University(Natural Sciences),2009,31(2):195-199. | |
| Authors: | LIU Xu-guang SHAO Shi-guang |
| Institution: | 1. Key Laboratory of Applied Biotechnology of Lianyungang, Lianyungang Teachers College, Lianyungang 222006, China; 2. The School of Life Sciences, Nanjing University, Nanjing 210093, China; 3. College of Life Sciences, Nanjing Normal University, Nanjing 210046, China |
| Abstract: | Four phenol-degrading bacterial strains,which could use phenol as the sole carbon source for growth,were enriched,isolated and screened from wastewater samples collected from chemical plant etc.The phenol hydroxylase(LmPHs) gene-specific primers were designed,and the 641bp oligonucleotide fragments were produced by PCRamplification from three phenol-degrading strains.Phylogenetic analysis based on the 16SrRNAgene sequence of strain2N-17 showed that it belongs to the genus Pseudomonas.Compared with other methods,our methods showed the ability to characterize phenol degrading bacteria quickly,accurately and easily from the phenol polluted environments. |
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