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苦瓜叶片提取液对农杆菌生长抑制作用的初探
引用本文:单少杰,李翔,郭红梅,陈宏.苦瓜叶片提取液对农杆菌生长抑制作用的初探[J].天津师范大学学报(自然科学版),2014(1):81-82,96.
作者姓名:单少杰  李翔  郭红梅  陈宏
作者单位:[1]天津师范大学生命科学学院,天津300387 [2]天津师范大学天津市动植物抗性重点实验室,天津300387
基金项目:国家自然科学基金资助项目(31140028);天津市自然科学基金资助项目(11JCYBJC08400)
摘    要:采用不同质量浓度的苦瓜叶片乙醇提取液(3.1、6.3、12.5、25.0 g/L)处理农杆菌,分别在12、24、36、48 h测试其对农杆菌生长的影响.结果表明:苦瓜叶片乙醇提取液对农杆菌生长具有明显的抑制作用,随着提取液浓度的增加,抑菌效果增强;提取液的最佳处理质量浓度为6.3 g/L,最佳处理时间为24 h.

关 键 词:苦瓜叶片  提取液  农杆菌  抑菌作用

Inhibition of extract from the leaf of Momordica charantia L. on Agrobacterium
SHAN Shaojie,LI Xiang,GUO Hongmei,CHEN Hong.Inhibition of extract from the leaf of Momordica charantia L. on Agrobacterium[J].Journal of Tianjin Normal University(Natural Science Edition),2014(1):81-82,96.
Authors:SHAN Shaojie  LI Xiang  GUO Hongmei  CHEN Hong
Institution:(a. College of Life Science, b. Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, Tianjin 300387, China)
Abstract:The extract from the bitter melon leaves was made by anhydrous ethanol reflux method, and the different concentrations (3.1, 6.3, 12.5, 25.0 g/L) of extract were used to test the influence on the growth of Agrobacterium in 12, 24, 36, 48 h. The results showed that the extract from the bitter melon leaves can efficiently inhibit the growth of Agrobacterium. With the increase of the concentrations of extract, the antibacterial effect increased accordingly. The optimal concentration was 6.3 g/L, and the optimal processing period was 24 h.
Keywords:Momordica Charantia L  leaves  extract  Agrobacterium  antibacterial action
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