| 喜树嫩叶中喜树碱的HPLC提取方法的选择 |
| |
| 引用本文： | 李红英,龙澜,李亚杰,帅超群,程新华,黄建民,张亮.喜树嫩叶中喜树碱的HPLC提取方法的选择[J].湖北民族学院学报(哲学社会科学版),2012(2):139-141. |
| |
| 作者姓名： | 李红英 龙澜 李亚杰 帅超群 程新华 黄建民 张亮 |
| |
| 作者单位： | 湖北省农业科学创新中心鄂西综合试验站;恩施清江生物工程有限公司 |
| |
| 基金项目： | 2005年国家科技型中小企业技术创新基金项目 |
| |
| 摘 要： | 建立一种从喜树嫩叶中喜树碱的高相液相测定提取方法.采用Waters Symmetry C18(150×3.9 mm,5μm)高效液相色谱.乙腈∶水=35∶65(V/V)为流动相,1.0 mL/min的流速,检测波长254 nm,柱温:25℃,进样量10μl.以不同浓度的甲醇提取法与不同浓度的碱法作对比,结论:以80%甲醇为溶剂于50℃下超声提取1 h为最佳提取法.
|
| 关 键 词： | 喜树碱 HPLC 提取 |
Choice of HPLC Methods of Extracting CPT from the Tender Leaves of Camptotheca acuminate Decne |
| |
| LI Hong-ying,LONG Lan,LI Ya-jie,SHUAI Chao-qun,CHENG Xin-hua,HUANG Jian-min,ZHANG Liang.Choice of HPLC Methods of Extracting CPT from the Tender Leaves of Camptotheca acuminate Decne[J].Journal of Hubei Institute for Nationalities(Natural Sciences),2012(2):139-141. |
| |
| Authors: | LI Hong-ying LONG Lan LI Ya-jie SHUAI Chao-qun CHENG Xin-hua HUANG Jian-min ZHANG Liang |
| |
| Institution: | 1.West Comprehensive Experiment Station of Hubei Provincial Agriculture S&T Innovation Center Enshi 445000,China; 2.Enshi Qingjiang Bio-engineering Co.,LtD.,Enshi 445000,China) |
| |
| Abstract: | A method to extract the CPT from the tender leaves of Camptotheca acuminate Decne through HPLC.Using waters symmetry C18(150mm×3.9mm,5μm)HPLC.Acetonitrile: water =35:65(V/V) as the mobile phase,1.0mL/min flow rate,detection wavelength 254nm,column temperature:25℃,sampling quantity 10μl.With different degrees of methanol extraction with different concentrations of alkali method comparison.Conclusion:the best method is to choose methanol as the solvent at 80% to 50 degrees for 1 hours under ultrasonic extraction. |
| |
| Keywords: | Camptotheca acuminata HPLC extraction |
| 本文献已被 CNKI 等数据库收录！ |
|
