植物差异表达基因克隆技术及研究进展

随着分子生物学技术的深入发展,植物基因组研究已经由以全基因组测序为目标的结构基因组学转向以基因功能鉴定为目标的功能基因组学研究,目前分离并克隆差异表达基因已成为生命科学研究的热点.近些年来,国内外学者发展了多种分析差异表达基因的技术来研究植物在不同发育阶段、不同生理状态下的基因表达状况,掌握了生命活动过程中的重要信息.文章对建立在RNA水平上克隆植物未知差异表达基因的几种关键技术及其研究进展进行了综述,阐述了各技术的原理、技术路线、优缺点及相应的改进方法,并对它们在植物抗逆研究中的应用现状及前景作了展望.

Methods for Plant Differential Expressed Gene Cloning and Progress

The research of plant genome has been transferred from the structural genomics focusing on determining the complete sequences of the genome to the functional genomics focusing on elucidating the biological function of genes. Now cloning differential expressed genes is of prime interest in molecular biology. In the past ten years, some methods used for studying changes of gene expression in plants have been developed. The authors review the developments of techniques and studies for the plants related gene cloning in RNA level and expound their principles, technical routes, improved methods, advantages and defects. Moreover, their applications and prospects in plants related studies are discussed.