| 稠李的组织培养及无性系的建立 |
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| 引用本文： | 姜长阳,周晓丽,李萍,刘德利,宁淑香.稠李的组织培养及无性系的建立[J].辽宁大学学报(自然科学版),2003,30(4):384-387. |
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| 作者姓名： | 姜长阳 周晓丽 李萍 刘德利 宁淑香 |
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| 作者单位： | 1. 辽宁师范大学,生命科学学院,辽宁,大连,116029
2. 丹东职业技术学院,辽宁,丹东,118005 |
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| 摘 要： | 通过稠李的茎尖的分化增殖培养，不定苗的生长、生根、生根苗的移栽，以及无根苗的扦插等试验，筛选出稠李试管苗的增殖、生长、生根的培养基，以及试管苗的移栽和扦插基质．结果表明，MS BA0．6离体茎尖成活伸长理想培养基；MS BA0．5 IAA0．2 GA0．5培养基为稠李芽的增殖分化最适培养基；MS BA0．2 IAA0．1 GA2．0培养基是促进不定苗状苗生长的最适培养基；1/2MS IAA2．0是诱导生根的最佳培养基．河沙和炉灰渣是稠李生根试管苗移栽，以及无根试管苗扦插的理想基质．无根试管苗的扦插，可简化培养程序，提高繁殖系数．
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| 关 键 词： | 稠李 组织培养 无性系 增殖 生根苗移栽 无根苗扦插 樱桃砧木 |
| 文章编号： | 1000-5846(2003)04-0384-04 |
| 修稿时间： | 2003年1月8日 |
Tissue Culture and Establishment of Asexual Cell line of Prunus Padus |
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| JIANG Chang-yang,ZHOU Xiao-li,LI Ping,LIU De-li,NING Shu-xiang.Tissue Culture and Establishment of Asexual Cell line of Prunus Padus[J].Journal of Liaoning University(Natural Sciences Edition),2003,30(4):384-387. |
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| Authors: | JIANG Chang-yang ZHOU Xiao-li LI Ping LIU De-li NING Shu-xiang |
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| Institution: | JIANG Chang-yang~1,ZHOU Xiao-li~2,LI Ping~1,LIU De-li~1,NING Shu-xiang~1 |
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| Abstract: | The experiment studied the state of propagation, growth, radication and the tube seeding's transplantation and selected of suitable medium for propagation of the buds, the growth and radication of the explants. The results indicated that MS+BA0.6mg/L is the effective inducing medium. Medium contains MS+BA0.5mg/L+IAA0.2mg/L+GA0.5mg/L is the best differentiation medium for buds, 1/2MS+IAA0.2mg/L is the best radiation medium and sand or cinder is the suitable medium for transplanting or cuttage. Cuttage increases the efficiency of propagation. |
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| Keywords: | prunas padas asexual system propagation rooting transplantation |
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