| 拟穴青蟹GnRH-R的免疫识别 |
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| 引用本文： | 陶永,刘元婧,叶海辉,王桂忠,李少菁.拟穴青蟹GnRH-R的免疫识别[J].厦门大学学报(自然科学版),2011,50(5):799-802. |
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| 作者姓名： | 陶永 刘元婧 叶海辉 王桂忠 李少菁 |
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| 作者单位： | 厦门大学海洋与环境学院,福建厦门,361005 |
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| 基金项目： | 国家自然科学基金项目(41076081) |
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| 摘 要： | 促性腺激素释放激素(GnRH)及其受体(GnRH-R)对生殖具有重要的调控作用.迄今无脊椎动物GnRH及GnRH-R的研究尚少.本研究采用兔抗人GnRH-R、兔抗果蝇GnRH-R和兔抗海鞘GnRH-R的抗体,应用免疫印迹和免疫共沉淀技术对拟穴青蟹(Scyllaparamamosain)GnRH-R进行了免疫识别,所得结果如下:1)经免疫印迹检测,拟穴青蟹脑、胸神经团、视神经节和精巢中共有的免疫阳性条带分子质量为45~55 ku.脑和胸神经团中另有一条36 ku条带;在精巢中,兔抗人GnRH-R和兔抗果蝇GnRH-R抗体只有一条28 ku条带,而兔抗海鞘GnRH-R抗体显示有28,36和30 ku的条带.2)利用免疫共沉淀技术,分离出与GnRH-R抗体相结合的两种物质,分子质量分别为38.1和54.0 ku,分别与哺乳动物和非哺乳动物GnRH-R的分子质量接近.该发现提示拟穴青蟹体内存在GnRH-R,为深入了解甲壳动物生殖调控机理提供了理论依据.
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| 关 键 词： | GnRH-R 甲壳动物 免疫印迹 免疫共沉淀 |
Immunological Recognition of Gonadotropin-releasing Hormone Receptor (GnRH-R) in the Mud Crab,Scylla paramamosain |
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| TAO Yong,LIU Yuan-jing,YE Hai-hui,WANG Gui-zhong,LI Shao-jing.Immunological Recognition of Gonadotropin-releasing Hormone Receptor (GnRH-R) in the Mud Crab,Scylla paramamosain[J].Journal of Xiamen University(Natural Science),2011,50(5):799-802. |
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| Authors: | TAO Yong LIU Yuan-jing YE Hai-hui WANG Gui-zhong LI Shao-jing |
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| Institution: | TAO Yong,LIU Yuan-jing,YE Hai-hui,WANG Gui-zhong,LI Shao-jing(College of Oceanography and Environmental Science,Xiamen University,Xiamen 361005,China) |
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| Abstract: | Gonadotropin-releasing hormone(GnRH) and its receptor(GnRH-R)play an important role in the regulation of reproduction.To date,there has been little study on the GnRH and GnRH-R in invertebrates.In this study,with three GnRH-Rs of rabbit antibody against different species,including human,fruit fly and sea squirt,Western blotting and co-immunoprecipitation were applied to test the GnRH-R in mud crab,Scylla paramamosain.The common immunoreactive bands,with the molecular mass between 45 and 55 ku,appeared in th... |
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| Keywords: | gonadotropin-releasing hormone receptor(GnRH-R) Scylla paramamosain Western blot co-immunoprecipitation |
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