| 海洋生境枯草芽孢杆菌LHB02抗菌蛋白的分离纯化及部分特性分析 |
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| 引用本文： | 徐长安,毛勇.海洋生境枯草芽孢杆菌LHB02抗菌蛋白的分离纯化及部分特性分析[J].厦门大学学报(自然科学版),2011,50(5):922-927. |
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| 作者姓名： | 徐长安 毛勇 |
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| 作者单位： | 1. 国家海洋局第三海洋研究所,福建厦门,361005
2. 厦门大学海洋与环境学院,福建厦门,361005 |
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| 基金项目： | 海洋公益性行业科研专项(201005032); 厦门海洋研究开发院课题(K10102(1)) |
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| 摘 要： | LHB02是一株分离自海滩污泥的海洋生境枯草芽孢杆菌(Bacillus subtilis),其发酵产物对海水养殖常见致病菌弧菌有很强拮抗能力.采用硫酸铵沉淀、Sephadex G-50凝胶层析等方法,进一步对LHB02发酵产物分离纯化,获得了分子质量为47 ku单一条带的糖蛋白.经抗菌活性分析及稳定性测试发现,纯化后的抗菌蛋白对哈维氏弧菌(Vibrioharveyi)、溶藻弧菌(Vibrio alginolyticus)和鳗弧菌(Vibrio anguillarum)有很强的抑制作用,对热稳定及酸的耐受性也比较强(pH 3.0~8.0),但对部分蛋白酶敏感.LHB02所产抗菌蛋白的强抑菌作用和高稳定性,预示着该菌可作为一种生防细菌开发,用于大黄鱼养殖中弧菌病的防治.
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| 关 键 词： | 枯草芽孢杆菌 抗菌蛋白 分离纯化 |
Purification and Partial Characterization of Antibacterial Protein from Marine Bacillus Strain LHB02 |
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| XU Chang-an,MAO Yong.Purification and Partial Characterization of Antibacterial Protein from Marine Bacillus Strain LHB02[J].Journal of Xiamen University(Natural Science),2011,50(5):922-927. |
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| Authors: | XU Chang-an MAO Yong |
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| Institution: | XU Chang-an1,MAO Yong2(1.Third Institute of Oceanography,State Oceanic Administration,Xiamen 361005,China,2.College of Oceanography and Environmental Science,Xiamen University,China) |
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| Abstract: | The strain of LHB02 was a Bacillus subtilis isolated from marine sediment,and the crude antibacterial substance from its fermentation broth showed strong inhibitory activity against pathogenic vibrios.In present study,a kind of antimicrobial glycoprotein with molecular weight 47 ku was gained by further purification using ammonium sulfate precipitation combined with column chromatography on Sephadex G-50.In the following antimicrobial test,the purified antimicrobial protein showed strong antagonistic effect... |
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| Keywords: | Bacillus subtilis antibacterial protein purification |
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