HMBA对人成骨肉瘤MG-63细胞增殖和相关基因表达的影响

研究了环六亚甲基双乙酰胺对人成骨肉瘤MG-63细胞的增殖和相关基因表达的影响.实验结果表明HMBA可明显抑制MG-63细胞的增殖,细胞生长抑制率达50.69%,分裂指数抑制率达58.8%,增殖细胞核抗原的表达降低,细胞周期被阻滞在G0/G1期.免疫细胞化学染色结果显示,经HMBA处理之后,与增殖分化调控有关的癌基因c-myc、c-fos、c-erbB-2、mtp53的表达降低、抑癌基因p21WAF1/CIP1、p16、rb的表达升高.研究结果表明,HMBA能够有效抑制人成骨肉瘤MG-63细胞的增殖活动,其对细胞增殖的抑制作用与HMBA下调c-myc、c-fos、c-erbB-2、mtp53等癌基因以及上调p21WAF1/CIP1、p16、rb等抑癌基因的表达,从而调控细胞周期有重要关系.

The Effects of HMBA on Proliferation and Associated Genes Expression of Human Osteosarcoma MG-63 Cells

In this paper, we studied the effects of HMBA on the proliferation and expression of genes in human osteosarcoma MG- 63 cells. The experiment results showed that after treated with 5 mmol/L HMBA, the proliferation rate of MG-63 cells was inhibited, the inhibition rate amounts to 50. 69%, mitotic index was reduced from 2. 712% to 1. 117%, and its inhibition rate amounts to 58. 8% ,the expression level of PCNA was reduced after treatment as well,and the cell cycle was arrested at G0/G1 phase. The immunocytochemistry assay also revealed that the expression level of oncogene including c-myc,c-fos, c-erbB-2 was downregulated, and expression level of tumor suppressor gene including p21 ,p16 ,rb was upregulated. In conclusion,the result of this study showed that the HMBA could effectively inhibit the proliferation activity of the human ostosarcoma MG-63 cells, cause the arrest of G0/G1 phase. The primary results revealed that the mechanism of HMBA was related to cell cycle regulation by downregulating the expression level of c-myc, c-los, c-erbB-2, mtp53 and upregulating the expression level of p21, p16. rb .