| 花生根瘤菌转吸氢基因的研究 |
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| 引用本文： | 龙敏南,刘广发.花生根瘤菌转吸氢基因的研究[J].厦门大学学报(自然科学版),1996,35(2):248-251. |
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| 作者姓名： | 龙敏南 刘广发 |
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| 摘 要： | 以ｐＲＫ２０７３为辅助质粒、通过三亲本杂交，将携带豌豆根瘤菌吸氢基因的质粒ｐＡＬ６１８转移到花生根瘤菌ＮＢ２中，通过抗性筛选、质粒检测及吸氢活性测定，获得一系列能稳定遗传的结合株，其中ＮＢ２－７，ＮＢ２－１１在自生条件下其吸氢活性为受体株的１５倍，在共生条件下其吸氢活性为受体株的２～３倍．
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| 关 键 词： | 花生根瘤菌，吸氢基因，吸氢酶 |
Studies on Translation of Hydrogen Uptake Genes into Rhizobium arachis |
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| Long Minnan, Liu Guangfa ,Zhang Fengzhang, Xu Liangshu.Studies on Translation of Hydrogen Uptake Genes into Rhizobium arachis[J].Journal of Xiamen University(Natural Science),1996,35(2):248-251. |
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| Authors: | Long Minnan Liu Guangfa Zhang Fengzhang Xu Liangshu |
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| Institution: | Dept.of Biol. |
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| Abstract: | Transfer of plasmid pAL618 containing Hydrogen uptake genes from E. coli into Rhizthem arachis NB; was carried out by triparental mating system; using pRK2073 as the helper plasmid. Rhizobium arachis transconjugants carrying pAL618 were selected in Rhizobium minimal medium containing tetracycline (10 mg/L). The expression of hydrogenase activities of transconjugants NB2-7 and NB2-11 was studied in free-living and symbiotic state. The hydrogenase activities increased by 15-fold in comparison with recipient strain NB2 in free-Living state and 2-3 fold in symbiotic state. The plasmld electropheresis profile showed that transconjugants have harbored the pAL618. |
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| Keywords: | Rhizobium arachis Hydrogen uptake genes Hydrogenase |
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