不同作物间共用SSR引物的初步研究

根据相关报道,利用互联网在GENE BANK中查询油菜的简单重复序列及其两端序列来设计引物,并合成了2对甘蓝型油菜的SSR引物（Bn92A,Bn72A),通过所建立的降温PCR（Touchdown PCR)方法,对来自禾本科,十字花科,芸香科,锦葵科等12种经济作物的DNA进行PCR扩增。结果显示,PCR扩增产物条带清晰。所有12份材料用2对SSR引物扩增出的主带片段大小一致,分别为150bp(Bn92A),250bp(Bn72A）。这与文献报道在甘蓝型油菜中SSR扩增出的片段大小完全一致。本研究说明,各物种基因组间存在着相当大的相似性,这2对甘蓝型油菜的SSR引物可在其它经济作物分子标记的基因组比较作图应用。

Common SSR Primers for Molecular Marker in Different Crops

Using 2 pairs of SSR(Simple Sequence Repeats) primers form Brassica napus-Bn92A?Bn72A, synthesized according to the reports on GENE BANK, DNAs extracted from 12 different crops (rice, maize, cotton, rape, orange, cabbage, et al.) were amplified by "touchdown" PCR.The result shows that, the main bands of 150 bp and 250 bp amplified by Bn92A?Bn72A respectively appear in all 12 crops and are the same size in Brassica napus by the same SSR primers. The results indicated that different kinds of genomes of crops have the certain extent comparability, and can use the common SSR primers for the molecular markers on genome comparative mapping.