| 应用小鼠胸腺细胞法与脾细胞法测定IL—2活性的比较 |
| |
| 引用本文： | 梁基选,周海虹.应用小鼠胸腺细胞法与脾细胞法测定IL—2活性的比较[J].厦门大学学报(自然科学版),1998,37(2):293-297. |
| |
| 作者姓名： | 梁基选 周海虹 |
| |
| 作者单位： | 厦门大学抗癌研究中心，海外教育学院 |
| |
| 基金项目： | 国家自然科学基金,厦门大学肿瘤细胞工程国家教委开放研究室开放基金 |
| |
| 摘 要： | 分别应用ＣｏｎＡ激活的小鼠胸腺细胞增殖法和脾细胞增殖法对样品内白细胞介素－２（ＩＬ－２）的活性进行生物学测定．细胞增殖状况以３Ｈ－ＴｄＲ掺入强度显示，并对实验条件进行了探讨．结果表明；添加氢化考的松（ＨＣ）的胸腺细胞法适用于检测培养物上清内的ＩＬ－２活性，其实验操作较脾细胞法更为省时省事
|
| 关 键 词： | 白细胞介素－2，生物学测定，小鼠胸腺细胞，氢化考的松 |
A Comparison Between Murine Thymocytes and Splenocytes in Their Application in Assay for Interleukin 2(IL 2) |
| |
| Liang Jixuan,Zhou Haihong,Hu Weihong.A Comparison Between Murine Thymocytes and Splenocytes in Their Application in Assay for Interleukin 2(IL 2)[J].Journal of Xiamen University(Natural Science),1998,37(2):293-297. |
| |
| Authors: | Liang Jixuan Zhou Haihong Hu Weihong |
| |
| Institution: | Liang Jixuan 1 Zhou Haihong 2 Hu Weihong 3 |
| |
| Abstract: | Concanavalin A(Con A) activated murine thymocytes and splenocytes were used and compared in the biological assay for interlukin 2 (IL 2).Cell proliferation rate was detected by 3H TdR incorporation and shown as cpm value.The conditions of assys were investigated. The results indicate that thymocyte method with the addition of hydrocortisone (HC) is suitable for the detection of IL 2 activity in the supernatant of cell culture medium.Its experimental procedure is relatively simple and timesaving,as compared with the splenocyte method. |
| |
| Keywords: | Interleukin 2 Biological assay Murine thymocytes Hydrocortisone |
| 本文献已被 CNKI 维普 等数据库收录！ |
