大黄鱼养殖群体遗传多样性的同工酶

应用聚丙烯酰胺凝胶电泳（ＰＡＧＥ）法对取自厦门市火烧屿养殖大黄鱼的９种同工酶１５个基因座位进行分析，结果表明：大黄鱼眼球中乳酸脱氢酶（ＬＤＨ）酶谱表现为ＬＤＨ的经典模式．在所研究的大黄鱼养殖群体中，多态位点比例（Ｐ）为０．０６７，平均杂合度的观测值（Ｈｏ′）为０．０１３，预期值（Ｈｅ）为０．０１２，位点有效等位基因数（Ｎｅ）为１．０６７，Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ遗传偏离指数（Ｄ）为０．０８３．该养殖群体的遗传多样性水平较低，这与大黄鱼养殖群体已出现的种质退化是一致的

Genetic Diversity of Cultured Pseudosciaena crocea (Richardson) Stock by PAGE

Genetic diversity of farmed Yellow large croaker Pseudosciaena crocea stock collected from HuoShao island in Xiamen in May 29,1998 was investigated by polyacrylamid gel electrophoresis(PAGE). The genetic diversity was relatively low, with 6.7% of the polymorphism and 0.013 of the mean heterozygosity, while a polymorphic locus (IDDH 1) was detected. These results showed the genetic variation has seriously lost in farmed Yellow large croaker stocks.Also, the classical mode of LDH of animals was found in the crystalline lens of Yellow large croaker, which is deferent from some species of Sciaenidae like Nibea miichthioides . This result can be well utilized in the taxology.