多重荧光PCR同时检测转基因成分35S和Nos方法的建立

根据商品化转基因作物中常用的花郴花花叶病毒启动子（CaMV35S）和根癌农杆菌终止（Nos）的序列特点，设计并合成了两对引物和相对应的荧光双链探针，建立一种应用荧光双链探针的多重荧光PCR同时检测转基因成分35S启动子和Nos终止子的方法，并利用该方法对马铃薯、大豆、玉米、甜椒、番茄等11份实物样品进行了检测，其中有5份样品结果阳性，结果表明所建立的多重荧光PCR方法能同时检测了35S方法同时检测出35S和Nos双组分，较常规PCR技术更为简便、快速、准确，有很很好的应用前景。

Multiplex Fluorescence PCR Method for Detecting Transgenic Component 35S and Nos Simultaneously

The article is to establish Multiplex Fluorescence PCR (MF PCR) method for detecting transgenic component 35S promoter derived from Cauliflower Mosaic Virus and Nos terminator derived from Agrobacterium tumefaciens simultaneously. According to the specific sequence of 35S and Nos which have been used in transgenic crops frequently, two pairs of primers and two pairs of corresponding fluorophore double chain probes were designed and synthesized for detection of 35S & Nos simultaneously with MF PCR in a tube. 11 samples were tested with this method. The results showed that 5 samples were positive, 6 samples were negative. The described methods enabled a sensitive?specific?simple and accurate detection of genetically modified food and thus provide a useful tool for routine analysis of raw and processed food products.