| 胰蛋白酶抑制剂抗虫基因转化烟草的研究 |
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| 引用本文： | 吴晓梅,陈德海,缪颖,杨盛昌.胰蛋白酶抑制剂抗虫基因转化烟草的研究[J].厦门大学学报(自然科学版),2004,43(5):706-709. |
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| 作者姓名： | 吴晓梅 陈德海 缪颖 杨盛昌 |
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| 作者单位： | 厦门大学生命科学学院,植物基因与基因技术研究所,福建,厦门,361005 |
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| 基金项目： | 福建省科技项目(99 Z 105)资助 |
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| 摘 要： | 实验用携带质粒pAM194/TI的根癌农杆菌LBA4404转化烟草,通过筛选,共获得35株卡那霉素抗性苗.GUS组织染色、PCR检测及抗虫试验表明胰蛋白酶抑制剂抗虫基因已经成功地整合进再生植株染色体基因组中,有些已正确表达.
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| 关 键 词： | 转基因 烟草 胰蛋白酶抑制剂 根癌农杆菌介导法 |
| 文章编号： | 0438-0479(2004)05-0706-04 |
| 修稿时间： | 2003年7月2日 |
Studies on TI Gene Transformation to the Tobacco Plants |
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| WU Xiao-mei,CHEN De-hai,MIAO Ying,YANG Sheng-chang.Studies on TI Gene Transformation to the Tobacco Plants[J].Journal of Xiamen University(Natural Science),2004,43(5):706-709. |
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| Authors: | WU Xiao-mei CHEN De-hai MIAO Ying YANG Sheng-chang |
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| Abstract: | A sweet potato trypsin inhibitor(TI) gene was introduced into tobacco plants(Nicotiana tabaccu)by Agrobacterium tumefaciens-mediated leaf disc transformation.By plant tissue culturing and differentiation induction,35 kanamycin-resistant tobacco plant individuals were selected.GUS expression test and PCR amplification analysis showed that GUS reported gene and TI gene were successfully integrated into genome of all 35 independent kanamycin-resistant tobacco plant individuals and were normally expressed.In insecticidal bioassays of TI-transgenic tobacco plants,larval,the tobacco cutworm,was severely retarded while the control plants were vulnerable to it.This observation implied that Agrobacterium tumefaciens-mediated transformation and expression of sweet potato trypsin inhibitor can provide an efficient method for tobacco crop protection and production. |
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| Keywords: | transgene tobacco trypsin inhibitor Agrobacterium tumefaciens-mediated transformation |
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