| 钙调素依赖性的环核苷酸磷酸二酯酶的制备 |
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| 引用本文： | 丁文勇,任凤,赵宝昌.钙调素依赖性的环核苷酸磷酸二酯酶的制备[J].吉林大学学报(理学版),1999(3). |
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| 作者姓名： | 丁文勇 任凤 赵宝昌 |
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| 作者单位： | 大连医科大学生化教研室,大连,116027 |
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| 摘 要： | 通过两次离子交换柱层析， 从牛脑中制备钙调素依赖性的环核苷酸磷酸二酯酶． 所得酶不含内源性钙调素， 能被足量外加的钙调素激活 １４ 倍， 可用于测定细胞中的钙调素含量
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| 关 键 词： | 环核苷酸磷酸二酯酶 钙调素 制备 |
Preparation of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase |
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| Ding Wenyong,Ren Feng,Zhao Baochang.Preparation of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase[J].Journal of Jilin University: Sci Ed,1999(3). |
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| Authors: | Ding Wenyong Ren Feng Zhao Baochang |
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| Abstract: | Calmodulin dependent cyclic nucleotide phophodiesterase was prepared from bovine brain by two step DEAE cellulose column chromatography. The enzyme obtained was free of endogenous calmodulin and stimulated about 14 fold by exogenous calmodulin, it can be used to assay the content of cytosolic calmodulin. |
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| Keywords: | cyclic nucleotide phosphodiesterase calmodulin preparation |
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