HPLC法测定人参叶中人参黄酮苷的质量分数

采用高效液相色谱法,测定人参叶中的主要黄酮类成分人参黄酮苷山柰酚-3-O-β-D-半乳糖(2→1)-β-D-葡萄糖苷]的质量分数.色谱条件:Zorbax Extend C18柱(250mm×4.6mm,5μm),流动相为V(甲醇):V(乙腈):V(体积分数为0.1%的醋酸水溶液)=25:5:70,流速为1.2mL/min,检测波长268nm,色谱柱温度为25℃.结果表明:在进样量0.5～20.0μg范围内线性关系良好,相关系数r=0.9999(n=6);人参黄酮苷的平均回收率为98.5%;RSD=0.64%(n=6)。

Determination of Panasenoside in Leaves of Panax ginseng C.A.Meyer by HPLC

To establish an HPLC method for the determination of panasenoside ［kaempferol-3-O-β-D-glucopyranosyl-（1→2）-β-D-galactopyranoside］ in the leaves of Panax ginseng C.A.Meyer, the chromatographic separation was performed on a Zorbax Extend C18 column (250 mm×4.6 mm, 5 μm), with V(methanol) ∶V(acetonitrile) ∶ V(0.1% acetic acid/water)=25 ∶5∶70 as mobile phase at a rate of 1.2 mL/min and 25 ℃. The detection wavelength was set at 268 nm. The results show that the linearity of panasenoside was in a range of 0.5～20 μg(r=0.999 9, n=6), and the averagerecovery and RSD were 98.5% and 064% respectively (n=6).