用响应面法优化酶水解法制备葵花盘小分子肽

为提取并利用葵花盘中具有降尿酸和溶解痛风石作用的小分子肽, 以小分子肽得率为指标, 通过酶水解法优化提取条件, 确定碱性蛋白酶为最理想的酶. 通过单因素实验考察料液比、 时间、 温度、 pH值和加酶量对小分子肽得率的影响, 利用响应面Box-Behnken实验, 优化酶水解法提取葵花盘小分子肽的工艺, 得到其最佳工艺条件为： 加入碱性蛋白酶, m(料)∶V(液)=1∶15, 酶的用量为2%, pH=9.0, 温度为55 ℃, 时间为2 h, 葵花盘小分子肽的得率最高为(42.41±0.01)%.

Preparation of Small Molecular Peptides from SunflowerCalathideby Enzymatic Hydrolysis Method Using Response Surface Method#br#

In order to extract and utilize small molecularpeptides from the sunflower calathide that could reduce uric acid and dissolve tophi, the extraction conditions were optimized by the enzymatic hydrolysis method with the yield of small molecular peptides as the index, and the alkaline protease was determined to be the most ideal enzyme. The effects of material to liquid ratio, time, temperature, pH value and enzyme amount on the yield of small molecular peptides were investigated by single factor test. The response surface Mask Behnken test was used to optimize the extraction of small molecular peptides from sunflower calathide by enzymatic hydrolysis method.The results show that the optimum technological conditions are adding alkaline protease, m(material)∶V(liquid)=1∶15, the amount of enzyme is 2%, the pH=9.0, the temperature is 55 ℃, and the time is 2 h. The highest yield of small molecular peptides from sunflower calathide is (42.41±0.01)%.