| Se-scFv-B3对过氧化氢诱导的大鼠乳鼠心肌细胞损伤的保护作用 |
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| 引用本文： | 苏家明,魏景艳,宋宇,杨蕾,霍锐.Se-scFv-B3对过氧化氢诱导的大鼠乳鼠心肌细胞损伤的保护作用[J].吉林大学学报(理学版),2010,48(1):146-151. |
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| 作者姓名： | 苏家明 魏景艳 宋宇 杨蕾 霍锐 |
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| 作者单位： | 1. 吉林大学 药学院, 长春 130021； 2. 吉林大学 生命科学学院, 长春 130021；3. 吉林大学 畜牧兽医学院, 长春 130062； 4. 吉林大学 中日联谊医院, 长春 130033 |
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| 摘 要： | 用H2O2损伤大鼠乳鼠的心肌细胞建立氧化应激损伤模型, 考察谷胱甘肽过氧化物酶模拟物Se-scFv-B3对H2O2诱导的大鼠乳鼠心肌细胞氧化损伤的影响. 结果表明, Se-scFv-B3能部分增加心肌细胞存活率, 减少细胞凋亡, 恢复线粒体膜电位, 下调Caspase-3活力并降低细胞内活性氧的含量. 表明Se-scFv-B3可以保护心肌细胞抵制H2O2诱导的氧化应激损伤.
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| 关 键 词： | 氧化应激 凋亡 Se-scFv-B3 心肌细胞 过氧化氢 |
| 收稿时间： | 2009-05-26 |
Se-scFv-B3 Protecting Neonatal Rat Cardiac againstH2O2 Triggered Injury |
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| SU Jia ming,WEI Jing yan,SONG Yu,YANG Lei,HUO Rui.Se-scFv-B3 Protecting Neonatal Rat Cardiac againstH2O2 Triggered Injury[J].Journal of Jilin University: Sci Ed,2010,48(1):146-151. |
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| Authors: | SU Jia ming WEI Jing yan SONG Yu YANG Lei HUO Rui |
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| Institution: | 1. College of Pharmaceutical Science, Jilin University, Changchun 130021, China;2. College of Life Science, Jilin University, Changchun 130021, China;3. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;4.China Japan Union Hospital, Jilin University, Changchun 130033, China |
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| Abstract: | To investigate the antioxidant effect of Se-scFv-B3, a model system of H2O2 induced rat cardiac myocyte damage was established. Se-scFv-B3 was demonstrated to protect cardiac myocytes against H2O2 induced damage by the increase of cell viability, the decline of cell apoptosis, the inhibition of mitochondrial membrane potential (Δψm) collapse, the decrease of Caspase-3 activity and intracellular H2O2 after treatment of rat cardiac myocytes by Se-scFv-B3. |
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| Keywords: | oxidative stress apoptosis Se-scFv-B3 cardiac myocytes hydrogen peroxide |
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