4种含RGD的短肽对细胞粘附作用的影响

从两方面对比研究了4种含Arg-Gly-Asp(RGD)的细胞粘附肽RGD,RGDS,RGD-(NH₂)₂即RGE-NH_{2和RGDS-NH2对细胞粘附的影响. 一方面研究了固定在聚乙交酯丙交酯共聚物（PLGA）膜上的4种细胞粘附肽通过其细胞定向作用对大肠癌细胞（HCT-8）和人成骨肉瘤细胞（OS732）细胞粘附性 的促进作用; 另一方面研究了外源性细胞粘附肽对HCT-8和OS732在纤维连接蛋白（FN）上粘附的竞争性抑制作用. 结果发现，除了RGD-(NH2)2，其他3种肽都具有明显抑制细胞粘附的作用且具有一定的浓度依赖关系，其中以RGDS和RGDS-NH2的能力最强，RGD稍弱. 对于HCT-8细胞，3种肽的最大抑制率分别为53.3%， 56.3%，37.5%；而对OS732细胞，3种肽的最大抑制率分别为50.9%，49.8%，34%.}

Effects of Motif RGD-containing Small Peptides on Cell Adhesion

The cell adhesive effects of four peptides containing RGD,RGDS,RGD-(NH2)2 and RGDS-NH2 on Human Intestines Cancer(HCT-8),Human Osteogenic Sarconia(OS732) were investigated.First of all,these cell adhesion peptides were immobilized on PLGA blend membrane respectively and their effects on cell adhesion were investigated.The four peptides except RGD-(NH2)2 on the membrane can promote the cell adhesion to the membrane.On the other hand,MTT assay was used to examine the adhesion of HCT-8 cells and OS732 cells on the Fibronectin(FN) after treatment with cellular adhesion peptide,we found that cellular adhesion peptides RGDS,RGDS-NH2,RGD significantly inhibited the adhesion of HCT-8 cells and OS732 cells on the FN in dose-dependent manner.RGD-(NH2)2 does not show an obvious ability to inhibit cell adhesion.The maximum percentages of inhibition were 53.3%,56.3%,37.5% on HCT-8 and 50.9%,49.8%,34% on OS732 by RGDS,RGDS-NH2,RGD,respectively.