| 超声预处理对酶促拆分布洛芬反应的影响 |
| |
| 引用本文： | 安佰义,郑璐,吴卓夫,王磊,王智,陈光.超声预处理对酶促拆分布洛芬反应的影响[J].吉林大学学报(理学版),2016,54(5):1177-1180. |
| |
| 作者姓名： | 安佰义 郑璐 吴卓夫 王磊 王智 陈光 |
| |
| 作者单位： | 1. 吉林农业大学 园艺学院, 长春 130118； 2. 吉林大学 分子酶学工程教育部重点实验室, 长春130012;3. 吉林农业大学 生命科学学院, 长春 130118 |
| |
| 摘 要： | 通过考察超声预处理的超声功率、温度和时间,以及水活度和pH值等反应条件对酶促拆分布洛芬反应的影响,筛选得到超声预处理对酶促拆分布洛芬的最适反应条件.实验结果表明,超声预处理作用可大幅度提高酶的催化性能.
|
| 关 键 词： | 超声预处理 酶APE1547 布洛芬 立体选择性 酶活力 |
| 收稿时间： | 2016-03-24 |
Effects of Ultrasonic Pretreatment on Enzymatic Resolution of Ibuprofen |
| |
| AN Baiyi,ZHENG Lu,WU Zhuofu,WANG Lei,WANG Zhi,CHEN Guang.Effects of Ultrasonic Pretreatment on Enzymatic Resolution of Ibuprofen[J].Journal of Jilin University: Sci Ed,2016,54(5):1177-1180. |
| |
| Authors: | AN Baiyi ZHENG Lu WU Zhuofu WANG Lei WANG Zhi CHEN Guang |
| |
| Institution: | 1. College of Horticulture, Jilin Agricultural University, Changchun 130118, China;2. Key Laboratory of Molecular Enzymology and Engineering of Ministry
of Education, Jilin University, Changchun 130012, China;3. College of Life Science, Jilin Agricultural University, Changchun 130118, China |
| |
| Abstract: | We studied the effects of ultrasonic pretreatment power, pretreatment temperature, ultrasonic pretreatment time, and the reaction conditions of water activity and pH on the enzymatic resolution of ibuprofen, and obtained the optimum reaction conditions for the enzymatic resolution of ibuprofen. Experimental results show that the cataytic performance of enzyme can be greatly improved by ultrasonic pretreatment. |
| |
| Keywords: | ultrasonic pretreatment APE1547 enzyme ibuprofen stereoselectivity enzyme activity |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《吉林大学学报(理学版)》浏览原始摘要信息 |
| 点击此处可从《吉林大学学报(理学版)》下载免费的PDF全文 |
|
