| 在固体基质表面制备具有催化活性的含酶分子多层膜 |
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| 引用本文： | 李文江,周兵,沙晓祥,孙长青,王子忱,赵慕愚,向前.在固体基质表面制备具有催化活性的含酶分子多层膜[J].吉林大学学报(理学版),2000(4):75-78. |
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| 作者姓名： | 李文江 周兵 沙晓祥 孙长青 王子忱 赵慕愚 向前 |
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| 作者单位： | 1. 吉林大学化学系,长春,130023
2. 长春工程学院化学系,长春,130021 |
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| 摘 要： | 在固体基质表面自组装一层功能分子层 ,通过静电相互作用 ,将辣根过氧化物酶( HRP)与双吡啶盐 ( Py C6 BPC6 Py)交替沉积 ,制得多层酶电极 .用紫外可见光谱法跟踪石英基片上的组装过程 .详细描述多层膜电极以亚甲绿 ( MGH)为电子中继体的电化学行为 ,并研究此传感器对过氧化氢的电催化还原反应
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| 关 键 词： | 辣根过氧化物酶 分子多层膜 电催化 |
| 修稿时间： | : |
Fabrication of Multilayer Film with Catalytic Activity on the Solid Substrate Surface |
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| LI Wen-jiang,ZHOU Bing,SHA Xiao-xiang,SUN Chang-qing,WANG Zi-chen,ZHAO Mu-yu,XIANG Qian.Fabrication of Multilayer Film with Catalytic Activity on the Solid Substrate Surface[J].Journal of Jilin University: Sci Ed,2000(4):75-78. |
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| Authors: | LI Wen-jiang ZHOU Bing SHA Xiao-xiang SUN Chang-qing WANG Zi-chen ZHAO Mu-yu XIANG Qian |
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| Abstract: | A new amperometric enzyme sensor has been produced based on ionic self assembly technique. The assembly process was monitored by ultraviolet visible spectroscopy on quartz slides. The diffusing mediator methylene green (MGH) as electron shuttle in a solution was able to transfer electrons successfully between the active center of the immobilized HRP and the electrode surface. The enzyme electrode was sensitive to the reduction of hydrogen peroxide. |
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| Keywords: | horseradish peroxidase multilayer films electrode |
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