| 松花粉的抗氧化作用 |
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| 引用本文： | 徐佳,张丽梅,王立成,隋天卓,王琪菲,王丽萍.松花粉的抗氧化作用[J].吉林大学学报(理学版),2017,55(6):1626-1630. |
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| 作者姓名： | 徐佳 张丽梅 王立成 隋天卓 王琪菲 王丽萍 |
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| 作者单位： | 1. 吉林大学 生命科学学院， 长春 130012; 2. 新时代健康产业（集团）有限公司, 北京 102206;3. 吉林大学 化学学院, 长春 130012 |
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| 摘 要： | 先对松花粉进行脱油、水提、冻干,获得水提物冻干品,再与缺陷型大肠杆菌菌液进行混合,稀释至终质量浓度为0.5,1.0,2.0mg/mL,喂食模式生物秀丽隐杆线虫(线虫).通过测定线虫的寿命、产卵、吞咽及运动能力,确定松花粉水提物对线虫无明显毒性作用;通过10mmol/L过氧化氢体外氧化压力诱导线虫,确定0.5,1.0,2.0mg/mL松花粉水提物对线虫体外氧化压力有显著保护作用.
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| 关 键 词： | 抗氧化 抗衰老 秀丽隐杆线虫 松花粉 |
| 收稿时间： | 2017-02-24 |
Antioxidation of Pine Pollen |
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| XU Jia,ZHANG Limei,WANG Licheng,SUI Tianzhuo,WANG Qifei,WANG Liping.Antioxidation of Pine Pollen[J].Journal of Jilin University: Sci Ed,2017,55(6):1626-1630. |
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| Authors: | XU Jia ZHANG Limei WANG Licheng SUI Tianzhuo WANG Qifei WANG Liping |
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| Institution: | 1. College of Life Sciences, Jilin University, Changchun 130012, China;2. China New Era Health Industry (Group) Co. Ltd., Beijing 102206, China;3. College of Chemistry, Jilin University, Changchun 130012, China |
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| Abstract: | We obtained the lyophilized products of water extracts by removing oil, water extract, freeze drying pine pollen. The water extracts was diluted with Escherichia coli OP50 to a final mass concentration of 0.5,1.0,2.0 mg/mL toculture Caenorhabditis elegans. By measuring the lifespan, oviposition, swallowing, locomotion of C.elegans, we determined that water extracts of pine pollen had no toxic effects on C.elegans. Through oxidative
stress induced by 10 mmol/L hydrogen peroxide, we determined that 0.5,1.0,2.0 mg/mL water extracts of pine pollen had a significant protective effect on oxidative stress of C.elegans. |
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| Keywords: | anti aging anti\ Caenorhabditis elegans oxidant pine pollen |
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