| 盐胁迫条件下酿酒酵母的代谢通量分析 |
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| 引用本文： | 李明达,陈霞,韩丹,莫新迎,赵长新.盐胁迫条件下酿酒酵母的代谢通量分析[J].吉林大学学报(理学版),2010,48(4):699-703. |
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| 作者姓名： | 李明达 陈霞 韩丹 莫新迎 赵长新 |
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| 作者单位： | 大连工业大学 生物与食品工程学院, 辽宁省发酵工程重点实验室, 辽宁 大连 116034 |
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| 基金项目： | 国家"十一五"科技支撑计划项目 |
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| 摘 要： | 用计算代谢通量的方法研究酿酒酵母(Saccharomyces cerevisiae)在高盐胁迫条件下的生理代谢.结果表明,在盐胁迫条件下,胞外海藻糖、甘油、乙醇和乳酸的质量浓度分别较常规培养条件下提高了24.44%,60.89%,23.32%和26.76%,而柠檬酸、琥珀酸和苹果酸的质量浓度分别较常规培养条件下降低了82.11%,50.37%和53.33%.代谢通量分析表明,在盐胁迫培养条件下糖酵解途径各支路的代谢产物通量均有所增加,而TCA循环中各代谢产物的通量均有所减少.
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| 关 键 词： | 酿酒酵母 盐胁迫 代谢流分析 |
| 收稿时间： | 2009-12-08 |
Metabolic Flux Analysis of Saccharomyces cerevisiae under Salt Stress Conditions |
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| LI Ming-da,CHEN Xia,HAN Dan,MO Xin-ying,ZHAO Chang-xin.Metabolic Flux Analysis of Saccharomyces cerevisiae under Salt Stress Conditions[J].Journal of Jilin University: Sci Ed,2010,48(4):699-703. |
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| Authors: | LI Ming-da CHEN Xia HAN Dan MO Xin-ying ZHAO Chang-xin |
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| Institution: | Key Lab of Fermentation Engineering of Liaoning Province, School of Bio &|Food Technology ofDalian Polytechnic University, Dalian 116034, Liaoning Province, China |
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| Abstract: | A method to calculate the metabolic flux of Saccharomyces cerevisiae metabolism was studied under salt stress conditions. The result shows that under salt stress, the contents of trehalose, glycerol, ethanol andlactate were increased by 24.44%, 60.89%, 23.32% and 26.76%, respectively, the contents of citrate, succinate and fumarate were decreased by 82.11%, 50.37% and53.33%, respectively. The metabolic flux analysis indicates that the metabolic product flux of each branch channel in EMP was raised at the culture condition of the salt stress, in contrast, each metabolic flux of TCA cycle was reduced. |
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| Keywords: | Saccharomyces cerevisiae salt stress metabolic flux analysis |
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