| 快速响应温敏凝胶及其在生物分离中的应用研究 |
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| 引用本文： | 冯霞,陈莉,董晶,李博林.快速响应温敏凝胶及其在生物分离中的应用研究[J].南开大学学报,2005,38(6):34-40. |
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| 作者姓名： | 冯霞 陈莉 董晶 李博林 |
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| 作者单位： | 天津工业大学材料科学与化学工程学院 天津300160 |
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| 基金项目： | 国家自然科学基金资助项目(20174027)，天津市自然科学基金资助项目(033610511) |
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| 摘 要： | 采用减小尺寸与加入C aCO3粒子作致孔剂两种方法,研究了如何提高共聚温敏凝胶P(N IPA-co-GM A-D ex)的响应速度.考察了不同尺寸和多孔凝胶的退溶胀动力学、再溶胀动力学,实验结果表明:减小凝胶尺寸和加入致孔剂对提高凝胶的温度响应速度是有效的.研究了P(N IPA-co-GM A-D ex)凝胶对牛血清蛋白(BSA)的浓缩分离效果,发现P(N IPA-co-GM A-D ex)凝胶对蛋白质溶液有较好的浓缩分离能力.在实验的基础上,设计了一套用于蛋白质提纯的机械模型,测试了其对蛋白质溶液的分离能力,证实了机械模型的可行性.
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| 关 键 词： | N-异丙基丙烯酰胺 葡聚糖 水凝胶 温度敏感 致孔剂 生物分离 机械模型 |
| 文章编号： | 0465-7942(2005)06-0034-07 |
| 收稿时间： | 11 23 2004 12:00AM |
| 修稿时间： | 2004年11月23 |
Fast Responsive Temperature-sensitive Hydrogel and Its Application on Bioseparation |
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| Feng Xia, Chen Li, Dong Jing, Li Bolin.Fast Responsive Temperature-sensitive Hydrogel and Its Application on Bioseparation[J].Acta Scientiarum Naturalium University Nankaiensis,2005,38(6):34-40. |
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| Authors: | Feng Xia Chen Li Dong Jing Li Bolin |
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| Institution: | School of Material Science and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160, China |
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| Abstract: | The responsive rate,reswelling and deswelling kinetics of the temperature-sensitive copolymerized P(NIPA-co-GMA-Dex) hydrogels were investigated by decreasing the size of hydrogels and using CaCO_3 particles as pore-foaming agent.The results showed that the responsive rate could be increased by decreasing the size of hydrogel and adding CaCO_3 particles as pore-foaming agent.Studies on the concentrating effect of the P(NIPA-co-GMA-Dex) hydrogel to the BSA solution show that P(NIPAco-GMA-Dex) hydrogels can concentrate BSA solution effectively,based on which,a set of mechanical appliance of gel-extraction separation of BSA was designed and its concentrating effect was tested. |
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| Keywords: | N-isoporpylacrylamide dextran hydrogel temperature-sensitive pore-foaming agent bioseparation mechanism model |
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