| HPLC-ELSD法测定胃泰咀嚼片中黄芪甲苷质量比 |
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| 引用本文： | 赵威,李云涛,杨波,杨书良.HPLC-ELSD法测定胃泰咀嚼片中黄芪甲苷质量比[J].哈尔滨商业大学学报(自然科学版),2012(3):271-273. |
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| 作者姓名： | 赵威 李云涛 杨波 杨书良 |
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| 作者单位： | 哈尔滨商业大学药学院;哈药集团世一堂制药厂 |
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| 摘 要： | 建立胃泰咀嚼片中黄芪甲苷的质量比测定方法.采用高效液相色谱-蒸发光检测器定量分析黄芪甲苷的质量比,使用Alltima-C18(5μm,4.6 mm×250 mm)色谱柱,流动相为甲醇-水(68∶32),流速1mL/min;蒸发光散射检测器(ELSD)飘移管温度50℃,载气压力1.58 Bar,放大系数(Gain)为10;进样量为20μL.表明黄芪甲苷在0.95～22.8μg之间呈良好的线性关系,r=0.999 00;加样回收率为98.97%,RSD为0.64%,在常温下8 h内稳定性较好.本方法简便快速,准确可靠,可作为胃泰咀嚼片的质量控制方法.
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| 关 键 词： | 胃泰咀嚼片 黄芪甲苷 HPLC-ELSD |
Determination of astragaloside in Weitai chewable tablets by HPLC-ELSD |
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| ZHAO Wei,LI Yun-tao,YANG Bo,YANG Shu-liang.Determination of astragaloside in Weitai chewable tablets by HPLC-ELSD[J].Journal of Harbin University of Commerce :Natural Sciences Edition,2012(3):271-273. |
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| Authors: | ZHAO Wei LI Yun-tao YANG Bo YANG Shu-liang |
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| Institution: | 1(1.School of Pharmacy,Harbin University of Commerce,Harbin 150076,China; 2.Shiyitang Pharmaceutical Factory of Harbin Pharmaceutical Group,Harbin 150088,China) |
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| Abstract: | To establish an method for the determination of astragaloside in Weitai chewable tablets.Conducted determination of astragaloside in this drug by HPLC-ELSD.Chromatogram condition: selected Alltima-C18(5 μm,4.6 mm×250 mm);mobile phase:methanol-water(68∶ 32),speed of flow was 1.0 mL/min;the ELSD conditions were as follows: pressure of compressed air 1.58 bar,drift temperature 50 ℃ and gain 10.The injection volume was 20 μL.The linear range of astragaloside was 0.95~22.8 μg(r=0.999 0) and the average recovery rate was 98.97%,RSD=0.64%,this substance in eight hours at room temperature stability was good.This method was accurate,reproducible and stable,and could be used for quality control of Weitai chewable tablets. |
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| Keywords: | Weitai chewable tablets astragaloside HPLC-ELSD |
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