| 不同培养条件和贮藏方法对皱皮木瓜花粉萌发率的影响 |
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| 引用本文： | 董美芳,陈明海,贾婉,张赛,张振兴.不同培养条件和贮藏方法对皱皮木瓜花粉萌发率的影响[J].河南大学学报(自然科学版),2012,42(3):291-294. |
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| 作者姓名： | 董美芳 陈明海 贾婉 张赛 张振兴 |
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| 作者单位： | 河南大学生命科学学院,河南开封,475004 |
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| 基金项目： | 河南省教育厅自然科学研究项目(2011B180002);河南省高校青年骨干教师资助计划(2009GGJS-152) |
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| 摘 要： | 采用离体培养法探讨了温度及蔗糖、H3BO3、CaCl2溶液浓度对皱皮木瓜花粉萌发率的影响,以及不同贮藏及解冻方法对花粉活力的影响.结果表明:皱皮木瓜花粉萌发的最适温度为20℃,最适蔗糖、H3BO3、CaCl2溶液浓度分别为150 g/L、20 mg/L和50 mg/L.在上述最佳单因子组合条件下,花粉萌发率高于任一单因子的促进作用.4℃预冷3h,-83℃超低温冷冻保存,37～40℃水浴快速解冻(4～5 min)后立即放于20℃水浴中的方法较有效地保持了花粉的活力.
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| 关 键 词： | 皱皮木瓜 离体培养 花粉保存 花粉活力 |
Effects of Different Culture Conditions and Storage Methods on Pollen Germination Rates of Chaenomeles speciosa |
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| DONG Mei-fang
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CHEN Ming-hai
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JIA Wan
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ZHANG Sai
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ZHANG Zhen-xing.Effects of Different Culture Conditions and Storage Methods on Pollen Germination Rates of Chaenomeles speciosa[J].Journal of Henan University(Natural Science),2012,42(3):291-294. |
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| Authors: | DONG Mei-fang CHEN Ming-hai JIA Wan ZHANG Sai ZHANG Zhen-xing |
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| Institution: | (College of Life Science,Henan University,Henan Kaifeng 475004,China) |
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| Abstract: | Effects of different culture conditions and storage or thawing methods on pollen germination rates of Chaenomeles speciosa in vitro were investigated.The results showed that the optimal temperature of pollen germination is 20 ℃,the most comfortable concentrations are respectively 150 g/L sugar solution,20 mg/L H3BO3 solution and 50 mg/L CaCl2 solution.And,in the best single factor combination conditions,pollen germination rate is higher than any single factor.-83 ℃ cryopreservation after precooling under 4 ℃ for 3 h,followed 37~40 ℃ water bath for 4~5 min and 20 ℃ water bath immediately,that maintains pollen vitality effectively. |
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| Keywords: | Chaenomeles speciosa culture in vitro pollen storage pollen vitality |
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