| 固相微萃取-气相色谱-质谱联用测定人血清中游离脂肪酸 |
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| 引用本文： | 张廉奉,邱东方,闫家伟,察冬梅.固相微萃取-气相色谱-质谱联用测定人血清中游离脂肪酸[J].河南大学学报(自然科学版),2009,39(5). |
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| 作者姓名： | 张廉奉 邱东方 闫家伟 察冬梅 |
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| 作者单位： | 1. 南阳师范学院,化学与制药工程学院,河南,南阳,473061
2. 中南民族大学,化学与材料科学学院,武汉,430074 |
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| 基金项目： | 河南省科技攻关计划项目(0821002270051);;南阳师范学院资助项目 |
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| 摘 要： | 采用甲基丙烯酸丁酯-羟基硅油(BMA/OH-TSO)固相微萃取探头(SPME)与气相色谱-质谱(GC-MS)联用测定人血清中游离脂肪酸的含量.用H2SO4-甲醇对血清中游离脂肪酸进行甲酯化反应,考察了影响探头萃取效率的因素如时间,温度和盐浓度等,优化了衍生化条件.结果表明:该方法检测限低,重现性好,线性范围宽(2~3个数量级),回收率在78.38%~105.1%之间,可用于血清中游离脂肪酸的测定.
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| 关 键 词： | 固相微萃取 气相色谱-质谱 衍生化 血清 游离脂肪酸 |
Determination of Free Fatty Acids in Human Serum Using Gas Chromatography-Mass Spectrometry Preceded by Solid-Phase Microextraction |
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| ZHANG Lian-feng,QIU Dong-fang,YAN Jia-wei,CHA Dong-mei.Determination of Free Fatty Acids in Human Serum Using Gas Chromatography-Mass Spectrometry Preceded by Solid-Phase Microextraction[J].Journal of Henan University(Natural Science),2009,39(5). |
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| Authors: | ZHANG Lian-feng QIU Dong-fang YAN Jia-wei CHA Dong-mei |
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| Institution: | 1.College of Chemistry and Pharmacy Engineering;Nanyang Normal University;Hena Nanyang 473061;China;2.College of Chemistry and Material Science;South-Central University for Nationalities;Wuhan 430074;China |
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| Abstract: | A novel solid-phase microextraction(SPME) fiber coated with butyl methacrylate/hydroxy-terminated silicone oil(BMA/OH-TSO) was applied to the analysis of free fatty acids(FFAs) in human serum samples by coupling to gas chromatography-mass spectrometry(GC-MS).The free fatty acids in human serum were esterified using H2SO4-methanol.Influences of related experimental parameters such as extraction time,temperature,and salt addition were systematically investigated.The derivatization parameters were optimized.Re... |
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| Keywords: | solid-phase microextraction gas chromatography-mass spectrometry derivatization human serum free fatty acids |
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