微囊化转CEA基因细胞诱导小鼠抗肿瘤免疫的研究

为发展癌胚抗原(CEA)阳性肿瘤的治疗性疫苗,应用基因工程和静电液滴技术制备出微囊化转CEA基因细胞,经腹腔免疫实验小鼠,再分别应用CEA基因转染的H 2 2 细胞小鼠皮下及腹腔接种,观察微囊化转CEA基因细胞对小鼠肿瘤的抑制作用及其诱导小鼠细胞毒性T淋巴细胞(CTL)特异性杀伤CEA 阳性肿瘤细胞的能力,并用流式细胞术检测了小鼠肿瘤细胞的生长周期和各实验组脾脏T淋巴细胞亚群的分布情况. 结果显示,微囊化转CEA 基因细胞可以有效抑制CEA阳性肿瘤的生长,诱导小鼠CTL对CEA阳性肿瘤细胞的特异性杀伤 ,并能改善荷瘤小鼠的免疫功能,延长腹水瘤小鼠的生存期.

CEA Gene Transfected Microencapsulated Cells Induced Tumor Specific Immunity

To explore the possibility of applying microencapsulated cells transfected with CEA gene as a vaccine on biological treatment of CEA positive tumors. CEA gene transfected Microencapsulated cells were prepared by droplet charge interaction technique and transplanted s.c. into mice. H 22 cells transfected with CEA gene were also inoculated i. p. (intradermal) into mice. The inhibiting effect of microencapsulated cells transfected with CEA gene on growth of tumor and its effect on inducing CTL to specific kill CEA positive tumor cells were evaluated. The growth cycle of tumor cells and distribution of T lymphocytes subgroups was analyzed by FCM. Microencapsulated cells transfected with CEA gene could effectively inhibit the growth of CEA positive tumor and to induce specific killing of CEA positive tumor cells. The cells could also improve the immunity function of mice and to expand the animal's life span.