| 含有重组人白细胞介素18基因的工程菌的产物表达和鉴定 |
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| 引用本文： | 董宇清,李荣贵,董春霞,赵进东,吴光耀.含有重组人白细胞介素18基因的工程菌的产物表达和鉴定[J].北京大学学报(自然科学版),2000,36(2):271-274. |
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| 作者姓名： | 董宇清 李荣贵 董春霞 赵进东 吴光耀 |
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| 作者单位： | 北京大学生命科学学院,北京,100871 |
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| 摘 要： | 0引言 1996年,Ushio等1]从人的肝脏细胞中克隆了人的白细胞介素18(IL-18) cDNA,并在大肠杆菌中得到了表达.IL-18是国际上最新获得的细胞因子2].
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| 关 键 词： | 人白细胞介素18 基因克隆 表达 |
Identification and Expression of the Product from Engineering Bacterium Containing Recombinant Human Interleukin-18(IL-18) Gene |
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| DONG Yuqing,LI Ronggui,DONG Chunxia,ZHAO Jindong,WU Guangyao.Identification and Expression of the Product from Engineering Bacterium Containing Recombinant Human Interleukin-18(IL-18) Gene[J].Acta Scientiarum Naturalium Universitatis Pekinensis,2000,36(2):271-274. |
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| Authors: | DONG Yuqing LI Ronggui DONG Chunxia ZHAO Jindong WU Guangyao |
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| Abstract: | The cDNA of human Interleukin\|18 was obtained by RT\|PCR from the mRNA of human peripheral blood cells.The DNA sequencing was performed and the DNA was cloned into expression vector pBV220.The overexpression was performed in E.coli HB101.The expressive product was purified through Sephadex G100 and identified by N\|terminal amino acid sequencing and Western blot. |
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| Keywords: | human interleukin\|18 (hIL\|18) gene cloning expression |
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