固定化过氧化物酶催化去除五氯酚反应装置和条件探讨

利用Fe3O4吸附-明胶包埋-交联固定过氧化物酶(HRP)催化去除五氯酚(PCP),在最佳反应条件的基础上,探讨了固定化酶重复使用过程中催化去除效果的变化规律,设计了酶催化连续反应装置,确定了反应装置适宜的流速条件及PCP去除效果,酶催化性能的稳定性,以及二级催化处理的效果.结果表明,该装置最佳运行流速约为3 mL/min,在此流速下可长时间连续处理含PCP废水;固定化HRP具有良好的催化稳定性;在一级催化反应装置的处理效果不满足排放标准的情况下,可进行二级处理, PCP总去除率可达92.7%,水中残留五氯酚浓度为0.98 mg/L,明显低于国家一级排放标准(5 mg/L).

Condition and Effect of Reactor for Removal of Pentachlorophenol Catalyzed by Immobilized HRP

Based on the optimum condition for removal of pentachlorophenol in water by using HRP immobilized by Fe_3O_4 sorption-gelatin embedding-cross linkage method as catalyzer, the reacting fitting and the removal efficiency were studied. The flow rate of PCP solution through the fitting was selected for better removal efficiency of Pentachlorophenol. The repetitive use of immobilized HRP for PCP removal, and the effect of secondary treatment of wastewater were also examined. The results indicate that the optimal flow rate of PCP solution for the removal efficiency of the reacting fitting is around 3?mL/min and the PCP removal rate is quite stable. Repetitive use of immobilized HRP for PCP removal is stable. Once the PCP removal efficiency of primary treatment couldn't meet the drainage standard for PCP concentration, secondary treatment is also feasible. The PCP removal rate of secondary treatment can reach 92.7%, and the residual concentration of PCP can reach 0.98?mg/L, which is under national first drainage standard (5?mg/L) evidently.