蚯蚓钙调素结合蛋白的研究

以赤子爱胜蚓为材料，通过ＤＥＡＥ－Ｆａｓｔ Ｆｌｏｗ离子交换层析、ＣａＭ－Ｓｅｐｈａｒｏｓｅ亲和层析，分离纯化得到蚯蚓钙调素结合蛋白（ＣａＭＢＰｓ）。纯化的ＣａＭＢＰｓ对ＣａＭ激活的环核苷酸磷酸二酯酶活性有抑制作用，而且这种抑制作用可通过加入过量的ＣａＭ达到完全恢复。ＳＤＳ－ＰＡＧＥ显示ＣａＭＢＰｓ有３条明显主带，在ＥＧＴＡ存在时表现分子量分别为６２，４９和３０ｋＤ。紫外扫描测定含量分别为７．１７

Studies on CaM binding Proteins from Earthworm

Calmodulin binding proteins were purified from earthworm (Eisenia Foetida) by a procedure involving DEAE Fast Flow,CaM Sepharose 4B chromatographies.The purified CaM binding proteins inhibited the activity of CaM dependent PDE and the degree of inhibition increased with augmentation of the CaMBPs.But this inhibition could be eliminated by adding an excess of earthworm CaM.This appeared to be the typical character of CaM binding Proteins.Three main bands of purified CaMBPs were shown on SDS PAGE with apparent molecular weight of 62,49 and 30kD.Their content percent was determined as 7.17%,7.31% and 51.8% by gel scaning respectively.Three kinds of CaMBPs were detected by biotinylated CaM overlay method.The assay of enzyme activity indicates that there was CaM dependent Ca 2+ ATPase activity,but no NAD kinase one.