| 有限稀释法筛选重组AcNPV病毒 |
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| 引用本文： | 钟劲.有限稀释法筛选重组AcNPV病毒[J].北京大学学报(自然科学版),1998,34(4):549-553. |
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| 作者姓名： | 钟劲 |
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| 作者单位： | 北京大学生命科学学院 |
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| 摘 要： | 有限稀释法筛选重组ＡｃＮＰＶ病毒钟劲胡美浩１）（北京大学生命科学学院，北京，１００８７１）关键词重组病毒；梯度稀释；筛选；测活中图分类号Ｑ３３２０引言昆虫杆状病毒表达系统是一高效表达外源基因的表达系统。用于基因工程表达外源基因的ＡｃＮＰＶ（ａｕｔｏｇ...
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| 关 键 词： | 重组病毒 筛选 昆虫 AcNPV病毒 有限稀释法 |
Screening Recombinant AcNPV with Methods of Limited Dilution |
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| Abstract: | Viral supernatant of co transfected sf9 cells is diluted to make cells infected by a single recombinant virus.Because only the recombinant virus express fibrinolysis active products,it can be screened by measuring activity of medium of transfected sf9 cells.After two turns of screening,we isolated the pure recombinant virus.Compared with the plaque assay usually used in Baculovirus expression system,this method is more convenient and efficient. |
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| Keywords: | recombinant virus gradient dilution screening activity measurement |
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