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中国人HLA-G1的cDNA克隆、序列分析、表达和蛋白质空间结构预测
引用本文:周建军,曲宏,吴才宏,韩卫东,李志伟,丰美福.中国人HLA-G1的cDNA克隆、序列分析、表达和蛋白质空间结构预测[J].北京大学学报(自然科学版),2002,38(2):212-220.
作者姓名:周建军  曲宏  吴才宏  韩卫东  李志伟  丰美福
作者单位:1. 北京大学生命科学学院北京,100871;2. 301医院血液科北京,100853;3. 内蒙古大学生命科学学院,呼和浩特,010021;4. 中国科学院动物研究所,北京,100080
摘    要:从中国人外周血单个核细胞、胎盘组织和肝癌组织等样品中克隆了包含完整HLA-G1读框的cDNA;与国外同行获得的该基因及其蛋白质序列比较分析表明,该基因虽然有着细微的种族特异性,但高度保守;并获得了它的截断型重组蛋白,根据蛋白一级结构和同源比较方法,模建了它及其与特异性受体KIR2DL4形成复合体的空间结构模拟,预测了它们之间相互作用的特征.

关 键 词:HLA-G1  免疫耐受  cDNA克隆  序列分析  表达  空间结    相互作用

cDNA Cloning,Sequence Analysis,Expression and Protein Structure Modeling of HLA-G1 from Chinese
ZHOU Jianjun\ QU Hong\ WU Caihong.cDNA Cloning,Sequence Analysis,Expression and Protein Structure Modeling of HLA-G1 from Chinese[J].Acta Scientiarum Naturalium Universitatis Pekinensis,2002,38(2):212-220.
Authors:ZHOU Jianjun\ QU Hong\ WU Caihong
Abstract:HLA\|G1,which is a newly defined non\|classical HLA class I molecule,plays an important role in mediating immunotolerance and protecting embryo and even some kinds of tumors from NK cells attacking.The full\|length coding sequences containing cDNA of HLA\|G1 were cloned from placenta,monocytes and liver cancer tissue of Chinese donors.Sequence analysis reveals that it is a highly conserved human gene with only two amino acid mutation sites compared to foreign nationality.Its truncated form was overexpressed in E.Coli and the recombinant proteins were purified with affinity chromatography.We predict its possible three\|dimensional structure and interaction with specific receptor KIR2DL4 by knowledge\|based computer modeling.
Keywords:HLA\|G1  immunotolerance  cDNA cloning  sequence analysis  expression  three\|dimensional structure  interaction
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