兔ES样细胞系的建立及其特性分析

报道从２３７枚家兔胚胎中建成７个可连续传代的ＥＳ样细胞系。建系条件为，使用小鼠原始胚胎成纤维细胞（ＭＥ）作饲养层，以含１０％胎年血清和１０％兔血清的ＤＭＥＭ／Ｆ１２为培养基，添加白血病抑制因子（ＬＩＦ）或上皮生长因子（ＥＧＦ），胚龄为９０，９６ｈ。该细胞系的细胞。在许多方面类似于小鼠ＥＳ细胞，具干细胞的形态特征，呈集落型生长，可连续传代并保持其形态特征，具有一定的自发分化和诱导分化的能力，悬浮培养

Establishment and Characterization of Rabbit ES like Cell Lines

Seven continous ES like cell lines were established from 237 rabbit embryos. 90 and 96 hours embryos were plated on ME(mouse embryonic fibroblast cells)feeder layer, using DMEM/F12 medium with 10% FBS and 10%rabbit serum supplemented with LIF or EGF. ES like cells isolated from these embryos shared many characteristics with mouse ES cells: morphology of stem cell,colonial growth, maintenance of an undifferentiated state through serial passage, ability to differentiate spontaneously or by induction, ability to form embryoid bodies in suspension culture. Using these cells as nucleid donor, nuclear transfer resulted in 11% blastocyst development.